中国农业科技导报 ›› 2021, Vol. 23 ›› Issue (12): 54-65.DOI: 10.13304/j.nykjdb.2020.0940

• 生物技术 生命科学 • 上一篇    下一篇

苎麻种质资源镉富集能力基因型差异研究

张英,马玉申,汪娅梅,刘泽航,许岳军,邢虎成*,揭雨成*   

  1. 湖南农业大学苎麻研究所, 湖南省草类作物种质创新与利用工程技术中心, 长沙 410128
  • 收稿日期:2020-11-06 接受日期:2021-04-11 出版日期:2021-12-15 发布日期:2021-12-22
  • 通讯作者: 邢虎成 E-mail: xinghucheng@hunau.edu.cn;揭雨成 E-mail: ibfcjyc@vip.sina.com
  • 基金资助:

    国家科技平台项目(NICGR2018-072);

    国家自然科学基金项目(31371704,31872877)

Genotype Differences in Cadmium Accumulation Ability of Ramie on Cadmium-polluted Field

ZHANG Ying, MA Yushen, WANG Yamei, LIU Zehang, XU Yuejun, XING Hucheng*, JIE Yucheng*   

  1. Hunan Engineering Technology Center of Grass Crop Germplasm Innovation and Utilization, Institute of Ramie, Hunan Agricultural University, Changsha 410128, China
  • Received:2020-11-06 Accepted:2021-04-11 Online:2021-12-15 Published:2021-12-22
  • About author:张英 E-mail: 376096039@qq.com

摘要: 苎麻是我国重要的纤维作物之一,是非常有前景的重金属镉污染土地修复植物。为进一步评价和鉴定苎麻种质镉富集的特性,筛选出优异的高镉富集能力苎麻种质,对农田镉污染条件下种植的269份苎麻种质资源的镉富集能力进行了研究。结果表明,269份苎麻种质资源镉积累量、富集系数和转运系数均存在显著差异,其变异系数分别为31.10%、20.19%和19.59%。57.25%的苎麻种质各器官镉积累量的分布规律为萝卜根>繁殖根>麻叶>麻骨>原麻>麻壳,37.17%种质的繁殖根镉积累量显著高于萝卜根,4.46%种质分布规律表现不一致。系统聚类分析表明,269份苎麻种质分成4类,Ⅰ~Ⅳ类分别含有7份、76份、139份和47份种质。第Ⅰ类苎麻种质各部位镉积累量显著高于其他类群。通过隶属函数法对269份苎麻种质地上部镉积累量、富集系数和转运系数进行综合分析,筛选出高镉富集能力的种质18份,分别是坡埝青叶苎2号、长沙苎麻、杨柳坝坐蔸麻、湘潭竹根家麻、祁东苎麻2号、南坡红花滇黔苎、坡结滇黔苎、乐业灰绿苎、隆回本地麻、浏阳苎麻2号、七宝山苎麻2号、木鱼苎麻1号、大庸黄壳麻、南坡楔基苎、川苎1号、赤麻、74-69和坡口苎麻,其地上部镉积累量平均值为657.55 μg·株-1,区间为436.60~942.91 μg·株-1,富集系数平均值为1.10,转运系数平均值为0.93。上述结果为苎麻镉修复专用品种的选育奠定了基础,同时也为苎麻修复镉污染农田提供参考。

关键词: 苎麻, 镉积累量, 富集系数, 转运系数, 镉富集能力

Abstract: Ramie is one of the important fiber crops in China, and it is a promising plant for repairing the heavy metals. In order to evaluate the cadmium accumulation ability of ramie, 269 ramie germplasm resources under the cadmium-polluted field were studied. The results showed that there were significant differences in cadmium accumulation, enrichment coefficient and transport coefficient in these ramie germplasm resources, and the coefficient of variation were 31.10%, 20.19% and 19.59 %, respectively. The distribution  of cadmium accumulation in various organs of 57.25% ramie germplasm was radish root > reproductive root > leaf > stick > fiber > steam bark. The cadmium accumulation in reproductive root of 37.17% germplasm was significantly higher than that of radish root, and 4.46% of ramie germplasm showed inconsistent distribution. The cluster analysis roughly revealed 4 groups including 7, 76, 139 and 47 accessions in group Ⅰ~Ⅳ, respectively. The germplasm resources in group Ⅰ contained the highest cadmium accumulation. The membership function method was used to comprehensively evaluate the indicators of cadmium accumulation ability, and 18 germplasms with high cadmium accumulation ability were selected. They were Ponian qingyezhu 2, Changsha zhuma, Yangliuba zuodouma, Xiangtan zhugenjiama, Qidong zhuma 2, Nanpo honghuadianqianzhu, Pojie dianqianzhu, Leye huilyuzhu, Longhui bendima, Liuyang zhuma 2, Qibaoshan zhuma 2, Muyu zhuma 1, Dayong huangkema, Nanpo xiejizhu, Chuanzhu 1, Chima, 74-69 and Pokou zhuma. The average cadmium accumulation was 657.55 μg·plant-1, the range was 436.60~942.91 μg·plant-1. The average enrichment coefficient was 1.10, and the average transport coefficient was 0.93. Above results provided theological basis for selecting the high cadmium accumulation ability ramie germplasm and reference for repairing cadmium-polluted field.

Key words: ramie, cadmium accumulation, enrichment coefficient, transport coefficient, cadmium accumulation ability