拟南芥抗菌核病突变体275-7的初步研究

doi:10.3969/j.issn.1008-0864.2010.02.15

›› 2010, Vol. 12 ›› Issue (2): 81-85.DOI: 10.3969/j.issn.1008-0864.2010.02.15

拟南芥抗菌核病突变体275-7的初步研究

李丹1,2,黄军艳2,张学江2,刘学群,覃瑞1,刘胜毅2

(1.中南民族大学生命科学院, 武汉 4300741|2.中国农业科学院油料作物研究所, 中国农业部油料作物生物学重点开放实验室, 武汉 430062)

收稿日期:2010-02-01 修回日期:2010-03-01 出版日期:2010-04-15 发布日期:2010-03-30
通讯作者: 刘胜毅,研究员,博士,主要从事植物抗病及分子生物学研究。Tel:027-86812896; E-mail:liusy@oilcrops.cn
作者简介:李丹,硕士研究生,研究方向为植物功能基因组学。E-mail:0703216lidan@163.com。
基金资助:
国家自然科学基金项目(30900934);国家863计划项目(2006AA10A112)资助。

Preliminary Studies on Sclerotinia sclerotiorum Resistant Mutant 275-7 of Arabidopsis thaliana

LI Dan1,2, HUANG Jun-yan2, ZHANG Xue-jiang2, LIU Xue-qun1, QIN Rui1, LIU Sheng-yi2

(1.College of Life Science, South Central University for Minority Nationalities, Wuhan 430074|2.Key Laboratory of Oil Crop Biology, Ministry of Agriculture|Oil Crops Research Institute, Chinese Academy of Agricultural Sciences, Wuhan 430062, China)

Received:2010-02-01 Revised:2010-03-01 Online:2010-04-15 Published:2010-03-30

摘要/Abstract

摘要：

草酸是核盘菌致病过程中产生的毒素因子。以菌核病菌毒素草酸（3 mmol/L）为筛选压,从1 000个拟南芥T-DNA插入突变体中筛选出了1个草酸不敏感突变体,命名为275-7。通过拟南芥活体接种对突变体275-7进行菌核病抗性鉴定,与野生型相比,突变体275-7对菌核病的抗性显著增强（P<0.05）;荧光定量PCR检测结果表明,275-7中茉莉酸途径的标志基因PDF1.2的表达量是野生型的12倍,而水杨酸途径的标志基因PR1基因的表达量与野生型比较无差异。推测拟南芥突变体275-7可能通过增强水杨酸介导的防卫反应,从而表现出对菌核病的抗性。

关键词: 拟南芥;突变体;菌核病 ;抗性;草酸

Abstract:

Oxalic acid is a toxin produced when plant is attacked by Sclerotinia sclerotiorum. An oxalic acid insensitive mutant named 275-7 was screened from the T-DNA insertion mutant library of Arabidopsis thaliana (about 1 000 lines) using 1/2 MS with 3 mmol/L oxalic acid. In vivo leaf inoculation test was undertaken, in order to assess the resistance to Sclerotinia sclerotiorum of mutant 275-7. The result indicated that mutant 275-7 exhibited a higher resistance to S. sclerotiorum than the wild type Arabidopsis (P<0.05). Fluorescence real-time quantitative PCR result showed that the expression of jasmonic acid (JA)-responsive marker gene PDF1.2 in 275-7 were significantly up-regulated (12 fold) in comparison with the wild type plants, but the expression level of salicylic acid (SA)-responsive marker gene PR1 was not significantly different from the wild type. This suggested that the enhanced resistance to S. sclerotiorum in mutant 275-7 might be mediated through JA signal pathway.

Key words: Arabidopsis thaliana, mutant, Sclerotinia sclerotiorum, resistance, oxalic acid

中图分类号:

S432.4+4

李丹1,2,黄军艳2,张学江2,刘学群,覃瑞1,刘胜毅2. 拟南芥抗菌核病突变体275-7的初步研究[J]. , 2010, 12(2): 81-85.

LI Dan1,2, HUANG Jun-yan2, ZHANG Xue-jiang2, LIU Xue-qun1, QIN Rui1, LIU Sheng-y. Preliminary Studies on Sclerotinia sclerotiorum Resistant Mutant 275-7 of Arabidopsis thaliana[J]. , 2010, 12(2): 81-85.

拟南芥抗菌核病突变体275-7的初步研究

Preliminary Studies on Sclerotinia sclerotiorum Resistant Mutant 275-7 of Arabidopsis thaliana

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