关键词: CBF耐逆性;可溶性糖;丙二醛;过氧化物酶

Abstract:

The CBF gene cloned from cotton was transformed into tobacco so as to study its impacts on tobacco. The PCR results showed that the CBF gene was successfully transferred into tobacco, and the transcription and expression of the exogenous CBF gene in transgenic tobacco were indentified by RT-PCR. Then the physiological indexes of stress resistance in the transgenic tobaccos were determined. The results showed that the soluble sugar content and POD activity of transgenic tobacco were significantly higher than the wild-type tobacco, reaching to 1.22~7.91 times and 3.35~5.73 times, respectively. And the MDA content was obviously lower than that of the wild type, taking 36.1% ~ 81.1% of the wild-type. These results indicated that CBF gene transformation had indeed improved the stress resistance of tobacco.

Key words: CBF, stress tolerance, soluble sugar, MDA, POD