关键词: 向日葵;生物学效应;碳离子注入;过氧化物酶同工酶

Abstract:

To study the influence of carbon ions implantation to traits of sunflowers (Helianthus annuus L.) and to breed high-quality mutant varieties, carbon ions were implanted into sunflower seeds in doses of 0 (control group), 1×1013, 5×1013 and 9×1013 with the energy to 6MeV. Through field culture and collected data analysis, the results showed that the seeds of sunflowers implanted with 5×1013 carbon germinate earlier with higher germination rate; their plant height, stem diameter, leaf number and maximum leaf area are larger than other groups during vegetative growth period; the yield of them are also significantly better than other groups. By analyzing the peroxidase isozyme of the seedlings leaves  in carbon ion implantation and control groups, some differences were found in zymograms between the 4 groups sunflowers, which indicated carbon ion implantation has changed the biochemical and genetic characteristics of sunflowers in some extent. Comprehensive analysis of above results showed implanting 5×1013 carbon ions can induce more excellent biological characters and has important significance to further select good varieties of sunflowers.

Key words: sunflower, biological effects, carbonium ion, peroxidase isozyme