ABA、MeJA和SA诱导下的荠菜CBF途径冷响应相关基因表达调控研究

doi:10.3969/j.issn.1008-0864.2010.06.13

›› 2010, Vol. 12 ›› Issue (6): 75-80.DOI: 10.3969/j.issn.1008-0864.2010.06.13

ABA、MeJA和SA诱导下的荠菜CBF途径冷响应相关基因表达调控研究

周明琦,吴丽华,沈忱,林娟

(复旦大学生命科学学院植物科学研究所, 遗传工程国家重点实验室, 上海 200433)

收稿日期:2010-08-09 修回日期:2010-08-26 出版日期:2010-12-15 发布日期:2010-10-27
通讯作者: 林娟,副教授,博士,硕士生导师,主要从事植物生物技术研究。E-mail:linjuan@fudan.edu.cn
作者简介:周明琦,博士研究生,研究方向为植物生物技术。E-mail:082023064@fudan.edu.cn。
基金资助:
国家863计划项目(2008AA10Z105);上海市基础研究重点项目(09JC1401700);国家转基因生物新品种培育重大专项(2008ZX08003-004)资助。

Study on the Regulation of Cold-responsive Genes in CBF Signaling Pathway from Capsella bursa Induced by |ABA、MeJA and SA

ZHOU Ming-qi, WU Li-hua, SHEN Chen, LIN Juan

(School of Life Sciences, Institute of Plant Biology, Fudan University, State Key Laboratory of Genetic Engineering,
Shanghai 200433, China)

Received:2010-08-09 Revised:2010-08-26 Online:2010-12-15 Published:2010-10-27

摘要/Abstract

摘要：

植物在长期进化过程中对低温形成了一系列的适应能力和抵抗能力；CBF(CRT binding factor)途径是植物冷响应机制中重要的组成部分,该途径中一系列相关基因的级联调控网络与许多植物信号分子关系密切。为了阐明植物信号分子对荠菜CBF途径的调控模式,选用十字花科植物荠菜（Capsella bursa-pastoris）为材料,采用RT-PCR方法,研究了常温条件下三种信号分子（ABA、MeJA、SA）对荠菜CBF途径抗冷相关基因CbICE53、CbCBF、CbCOR15a、CbCOR15b、CbCAX51、CbRCI35的表达调控作用,结果显示各种基因在三种信号分子的诱导下其表达谱呈现不同的特点,这一结果对进一步阐明植物信号分子在CBF途径调控网络中的作用具有重要意义。

关键词: 荠菜;抗冷;CBF;ABA;MeJA;SA

Abstract:

During the long evolution many plants have developed an in-built ability to adapt and resist low temperature stress. The CRT binding factor (CBF) pathway is one of the important cold responsive mechanism, in which a delicate and complex network regulated by a series of cold-associated genes closely related to various plant signaling molecules. In order to explore the regulation of plant signaling molecules on CBF pathway, Capsella bursa-pastoris was selected as research material in this paper. The regulation of abscisic acid (ABA), methyle jasmonate (MeJA) and salicylic acid (SA) on the expression of CbICE53, CbCBF, CbCOR15a, CbCOR15b, CbCAX51 and CbRCI35 in Capsella bursa-pastoris were studied by RT-PCR. The results showed that the expression patterns of these genes presented different characteristics under treatments with 3 signaling molecular, which had laid a foundation for further demonstrating the function of plant signaling molecules in the regulatory network of CBF pathway.

Key words: Capsella bursa, Cold-response, CBF, ABA, MeJA, SA

中图分类号:

Q756

周明琦,吴丽华,沈忱,林娟. ABA、MeJA和SA诱导下的荠菜CBF途径冷响应相关基因表达调控研究[J]. , 2010, 12(6): 75-80.

ZHOU Ming-qi, WU Li-hua, SHEN Chen, LIN Juan. Study on the Regulation of Cold-responsive Genes in CBF Signaling Pathway from Capsella bursa Induced by |ABA、MeJA and SA[J]. , 2010, 12(6): 75-80.

[1]	杨德鑫,周时荣,权瑞党,黄荣峰*. AP2/ERF蛋白调控植物的非生物胁迫应答机制[J]. , 2012, 14(6): 23-29.
[2]	钟贵买,伍林涛,王健美,杨毅,李旭锋*. 转录因子AtWRKY28亚细胞定位及在非生物胁迫下的表达分析[J]. , 2012, 14(5): 57-63.
[3]	左然,徐美玲,张东远. 拟南芥HCF243蛋白参与叶绿体PSⅡ稳定调控机制的研究[J]. , 2012, 14(4): 55-60.
[4]	徐涛,王磊. 植物中依赖于RNA的RNA聚合酶研究进展[J]. , 2011, 13(6): 46-53.
[5]	王淼1,2,王旭静2,唐巧玲2,王志兴2,王育青1. 高等植物绿色组织特异表达启动子研究进展[J]. , 2010, 12(2): 33-37.
[6]	张融雪,张治礼,张执金,黄荣峰. 植物低温信号的感知、转导与转录调控[J]. , 2009, 11(3): 5-11.
[7]	陈福禄,傅永福,林辰涛. CO/FT调节元件与植物开花时间调节研究进展[J]. , 2009, 11(2): 17-22.
[8]	王希挺,程治军,万建民. 植物茎端分生组织CLV/WUS信号途径的研究进展[J]. , 2008, 10(5): 1-7.
[9]	姚沁涛,张文蔚,刘莉,韩榕,简桂良,齐放军. Whirly转录因子对非寄主菌诱导水稻HR反应的负调控作用[J]. , 2008, 10(5): 53-58.
[10]	胡守景,张治礼,黄荣峰. 植物应答干旱胁迫的基因表达调控[J]. , 2008, 10(4): 1-6.

ABA、MeJA和SA诱导下的荠菜CBF途径冷响应相关基因表达调控研究

Study on the Regulation of Cold-responsive Genes in CBF Signaling Pathway from Capsella bursa Induced by |ABA、MeJA and SA

PDF

可视化

摘要/Abstract

引用本文

使用本文

参考文献

相关文章 10

编辑推荐

Metrics