关键词: 电子束;金花葵(Abelmoschus manihot);离子注入;花粉萌发率;生活力;花粉

Abstract:

In order to observe the effect of electron beam on germination rate of Abelmoschus manihots pollen and the effect of electron beam on germination rate of Abelmoschus manihots M1 pollens came from the seed infused by copper ion. Electron beam were irradiated into Abelmoschus manihots pollen in doses of 0 (i.e. control group) 300Gy, 400Gy, 500Gy, 600Gy, 800Gy and 1 000Gy with the BF-5 Linear accelerator. The pollen was cultured. The result showed that all electron beam irradiations in experimental dose range can inhibit the pollen germination. However, in the case when the pollen came from the seed infused by copper ion is the other way. The lower doses of electron beam had no significant effect on the M1 pollens from the seed implanted in dose of 1×1011 Cu2+/cm2 with the energy of 4MeV, but the germination rate of M1 pollens from the seed implanted were obviously increased in dose of 1×1012 Cu2+/cm2 with the energy of 4MeV. Comprehensive analysis of the above results showed that the pollen resistance of electron beam was increased by implanted Cu2+. It is of optimistic importance to select good varieties of Abelmoschus manihot.

Key words: electron beam, Abelmoschus manihot, ion implantation, pollen germination rate, viability, pollen