关键词: Ⅰ型普鲁兰酶;细菌;支链多糖

Abstract:

TypeⅠpullulanase typically hydrolyzes the α-1,6 glucosidic linkages of pullulan or branched oligosaccharides in an endo-way to produce maltotriose or liner oligosaccharides, respectively. This enzyme plays a very significant role in the food, chemistry and medical industries, because they can degrade different saccharides with other glycoside hydrolases producing various products. At present, studies on this enzyme focus not only on screening novel strains, but also on obtaining type Ⅰ pullulanase with proper properties to suit industrial production. This review summarizes the research progress on type Ⅰ pullulanase from bacteria in some fields, such as catalytic properties, separation and cloning of gene, producing enzyme by microorganism. The industrial application of this enzyme and prospect of its research are also discussed.

Key words: type Ⅰ pullulanase, bacteria, amylopectin