重离子诱变选育聚β-羟基丁酸酯高产菌株研究

doi:10.3969/j.issn.1008-0864.2012.02.15

›› 2012, Vol. 14 ›› Issue (2): 95-100.DOI: 10.3969/j.issn.1008-0864.2012.02.15

重离子诱变选育聚β-羟基丁酸酯高产菌株研究

张楠,张昺林,王婉如,徐俊泉,张东明,薛林贵

(兰州交通大学化学与生物工程学院, 兰州 730070)

收稿日期:2011-10-10 修回日期:2011-11-03 出版日期:2012-04-15 发布日期:2012-04-13
通讯作者: 薛林贵,教授,博士,研究方向为微生物生理与微生物工程。E-mail:xuelg@mail.lzjtu.cn
作者简介:张楠,硕士研究生,主要从事生物化工、微生物工程研究。E-mail:zhangnan19863@163.com。
基金资助:
甘肃省科技支撑计划项目(090NKCA079);国家自然科学基金项目(30870384);铁道部科技研究开发计划课题(2010Z001-E)资助。

Studies on Selecting High-yield Poly-β-hydroxybutyrate Producing Strain by Heavy-ion Irradiation

ZHANG Nan, ZHANG Bing-lin, WANG Wan-ru, XU Jun-quan, ZHANG Dong-ming, XUE Lin-gui

(College of Chemical and Biology Engineering, Lanzhou Jiaotong University, Lanzhou 730070, China)

Received:2011-10-10 Revised:2011-11-03 Online:2012-04-15 Published:2012-04-13

摘要/Abstract

摘要：

聚β-羟基丁酸酯(PHB)作为一种新型的生物可降解高分子材料,日益受到人们的广泛重视。利用12C6+离子束对一株聚β-羟基丁酸酯（PHB）产生菌——芽孢杆菌Z-3进行诱变,然后进行筛选,从大量突变株中最终选育出一株PHB高产菌株A11,其PHB产量达到1.404 g/L,是出发菌株的1.33倍。研究结果揭示,重离子诱变育种技术具有良好的诱变效果,是一种有效的微生物诱变育种方法和途径。

关键词: 聚β-羟基丁酸;重离子;诱变;选育;高产菌株

Abstract:

As a new biodegradable polymer materials, Poly-β-hydroxybutyrate (PHB) has attracted wide attention from people day by day. This study adopts heavy-ion mutation breeding technology, using 12C6+ ion beams to mutate the PHB producing strain-Bacillus Z-3, then screen a PHB high yielding strain A11 from a mount of mutant strains. Its PHB output can reach 1.404 g/L. This is 1.33 times as compared with the original strain. The results indicate that heavy-ion mutation breeding technology has good mutagenic effects. It is an effective method and way to microbial mutation breeding.

Key words: poly-β-hydroxybutyrate, heavy ion, mutagenesis, selective breeding, high-yield strain

中图分类号:

Q93-31
S182

张楠,张昺林,王婉如,徐俊泉,张东明,薛林贵. 重离子诱变选育聚β-羟基丁酸酯高产菌株研究[J]. , 2012, 14(2): 95-100.

ZHANG Nan, ZHANG Bing-lin, WANG Wan-ru, XU Jun-quan, ZHANG Dong-ming, XUE Lin-gu. Studies on Selecting High-yield Poly-β-hydroxybutyrate Producing Strain by Heavy-ion Irradiation[J]. , 2012, 14(2): 95-100.

重离子诱变选育聚β-羟基丁酸酯高产菌株研究

Studies on Selecting High-yield Poly-β-hydroxybutyrate Producing Strain by Heavy-ion Irradiation

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