关键词: 内含肽;生物传感器;诊断工具;激素受体;蛋白质相互作用

Abstract:

The development of biosensors for the analysis of complex biological problems is essential both for the study of human diseases and validation of potential drug molecules. A novel application of intein-mediated protein splicing technologies has led to the development of a biosensor. Inteins have played critical roles in design of many assays and biosensors enabling efficient and precise analysis of important biological processes, such as enzyme activity, hormone receptor, vitamins and protein-protein interactions. In this paper, we review the application and development of intein-based biosensors.

Key words: intein, biosensor, diagnostic tool, hormone receptor, protein-protein interaction