›› 1, Vol. 1 ›› Issue (1): 122-126.DOI: 10.3969/j.issn.1008-0864.2012.03.19

• 资源环境 生物药物 生物质转化 • 上一篇    下一篇

不同肥料对连作烟草根际土壤微生物及酶活性的影响

段玉琪1,陈冬梅2,晋艳1,王海斌2,杨宇虹1,尤垂淮2,田卫霞2,林文雄2   

  1. (1.云南省烟草农业科学研究院, 云南 玉溪 653100|2.福建农林大学农业生态研究所, 福州 350002)
  • 收稿日期:2012-02-07 修回日期:2012-03-31 出版日期:2012-05-11 发布日期:2012-06-15
  • 通讯作者: 林文雄,教授,博士生导师,主要从事作物栽培和生态学研究。E-mail: wenxiong181@163.com
  • 作者简介:段玉琪,副研究员,主要从事烟草栽培研究。E-mail: duanyuqi@sina.com。
  • 基金资助:

    云南省烟草专卖局(公司)科技项目(08A02,07A05,09YN003,2010YN05)资助。

Effect of Different Fertilizers on Continuous Tobacco Cropping Rhizospheric Soil Microorganisms and Enzyme Activities

DUAN Yu-qi1, CHEN Dong-mei2, JIN Yan1, WANG Hai-bin2, YANG Yu-hong1, YOU Chui-huai2, TIAN Wei-xia2, LIN Wen-xiong2   

  1. (1.Yunnan Academy of Tobacco Agricultural Sciences, Yunnan Yuxi 653100|2.Agro-ecological Institute, Fujian Agriculture and Forestry University, Fuzhou 350002, China)
  • Received:2012-02-07 Revised:2012-03-31 Online:2012-05-11 Published:2012-06-15

摘要:

烟草连作障碍是制约烟草产量与品质的关键因素。以连作12年的烟草土壤为对象,通过施用不同肥料, 研究了不同肥料对连作烟草产量、根际土壤微生物量、土壤酶活性的影响。结果表明:不同施肥处理对连作烟草产量及土壤微生物及酶活性的影响存在一定差异,施用农家肥有利于提高连作烟草产量。土壤微生物量分析结果显示,不同施肥处理后烟草根际土壤微生物量碳、氮及微生物呼吸强度依次为:农家肥>有机肥>氮肥>复合肥。与土壤营养循环相关酶活性的分析结果表明,不同施肥处理后烟草根际土壤中性磷酸酶、碱性磷酸酶、脲酶、蔗糖酶活性以有机肥处理后活性最大,而酸性磷酸酶则以农家肥处理后活性最高。与土壤生物抗逆性相关的多酚氧化酶、过氧化物酶、过氧化氢酶、脱氢酶活性分析的结果显示,不同施肥处理后烟草根际土壤抗性相关酶活性大小依次为:农家肥>有机肥>氮肥>复合肥。

关键词: 烟草;连作障碍;土壤微生物量;土壤酶活性

Abstract:

Problems caused by continuous tobacco cropping were critical obstacles restricting the yield and quality of tobacco leaf. The trial was conducted in a filed, where tobacco was continuously planted for 12 years. By applying different fertilizers, we studied the effect of different fertilizers on the yield, quality and rhizospheric soil microbial biomass, enzyme activity of the rhizosphere. The results indicated that different fertilization treatments had different effects on leaf yield, soil microorganism and enzyme activity in continuous cropping, and farmyard manure application was favorable to improve the leaf yield. The result of soil microbial biomass analysis showed that microbial biomass C and N, and microbial respiration intensity ranked as farmyard manure>organic fertilizer>nitrogen fertilizer>compound fertilizer after different fertilizer treatments. Furthermore, the activity of neutral phosphatase, alkaline phosphatase, urease and sucrase in tobacco rhizospheric soil was the highest with application of organic fertilizer, and farmyard manure application had the highest acid phosphate activity. In addition, the activity of soil enzyme (polyphenol oxidase, peroxidase, catalase, dehydrogenase) involved in resistance ranked as farmyard manure>organic fertilizer>nitrogen fertilizer > compound fertilizer.

Key words: tobacco, continuous cropping impediment, soil microbial biomass, soil enzyme activity

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