关键词: Bt cry1Ah基因, Bt cry1Ie基因, 2mG2-epsps基因, 转基因玉米

Abstract:

A plant expression vector pMUHUESGM harboring modified Bt cry1Ah gene, Bt cry1Ie gene and glyphosate-tolerant 2mG2-epsps gene was constructed. The foreign genes cassette of the vector pMUHUESGM was transferred into maize (Zea mays L.) immature embryonic calli by microprojectile bombardment. Twenty-four T0 regenerated plants were obtained with 2mG2-epsps gene as a selectable marker gene and glyphosate isopropylamine salt as a screening agent. PCR analysis showed that 20 plants had the integration of cry1Ah, cry1Ie and 2mG2-epsps genes. T0 and T1 generation plants molecular detection showed that foreign genes had been not only integrated into the maize genome,but also inherited and expressed stably over generations in maize. Bioassay revealed that transgenic events had high resistance to Asia corn borer. These results provided reference for breeding of new insect-resistant maize,moreover fragment transformation improved biosafety of genetically modified organisms.

Key words: Bt cry1Ah gene, Bt cry1Ie gene, 2mG2-epsps gene, transgenic maize