关键词: 丁香假单胞菌;铁离子螯合剂;8-羟基喹啉;荧光物质合成

Abstract:

The fluorescent pigment secreted by Pseudomonas syringae is a principal characteristic for bacteria classification, identification, and also one of potential virulence-related factors. In this study, we determined the impact of different concentrations of minimal medium components on production of P. syringae fluorescent pigment biosynthesis; and compared the influence of several iron-ion chelators on recovering biosynthesis ability of fluorescent pigment in pseudomonas syringaes and bacterial growth. The results indicated that ① concentration of iron-ion was the most significant impact-factor, which could influence colony fluorescence of P. syringae on KB medium, while the concentration of proteose peptone, K2HPO4 and MgSO4 could not obviously affect colony fluorescence. ②The production of P. syringae fluorescent pigments could get restored on KB plates and be significantly promoted in KB liquid when iron-ion specific chelator 8-hydroxyquinoline added at the concentration of 0.05 g/L, with a slight inhibition on bacterial growth. However, this recovery by 8-hydroxyquinoline could be reversed by adding FeCl3 at concentration of 0.05 g/L. ③Other metal-ion chelators, such as citric acid, tartaric acid sodium, trisodium phosphate and ethylenediaminetetraacetic acid disodium, could not restore P. syringae colony fluorescence. Therefore, adding iron-ion specific chelator 8-hydroxyquino (0.05 g/L) to KB medium was a more practical method for restoring colony fluorescence of P. syringae.

Key words: Pseudomonas syringae, iron-ion chelator, 8-hydroxyquinoline, biosynthesis of fluorescent pigment