关键词: 胶质芽胞杆菌；玉米秸秆；活菌数；微生物肥料

Abstract:

Bacillus mucilaginosus is an usual strain of microbial fertilizer. Main raw materials such as starch, sucrose, glucose, etc. are used in traditional fermentation of biological potassium fertilizer. With corn stalk as raw material, this paper compared fermentation of Bacillus mucilaginosus in starch and sucrose medium and corn stalk enzymatic hydrolysate medium; discussed the feasibility of taking corn stalk enzymatic hydrolysate as carbon source. The results showed that the viable number of 3.52×108 cfu/mL was obtained using corn stalk enzymatic hydrolysate as carbon source, which was 1.63 times of the starch and sucrose culture medium. The result of optimization experiment of corn stalk enzymatic hydrolysate medium showed that the best glucose concentration was 15 g/L and the viable number reached 3.95×108 cfu/mL. The result of 5 L fermenter experiment indicated that viable number reached 6.76×108 cfu/mL under the condition of rotation speed 250 r/min and pH 7.0~7.2, which was 1.71 times of shake flask culture. The results indicated that it was possible that instead of starch or sucrose, corn stalk hydrolysate can ferments Bacillus mucilaginosus, which provides a new way for utilization of corn stalk.

Key words: Bacillus mucilaginosus, corn stalk, viable bacteria number, microbial fertilizer