关键词: 草菇；原生质体；紫外诱变；化学诱变；双亲灭活；PEG融合

Abstract:

The protoplasts of Volvariella volvacea V23 and V3552 were prepared by enzymatic method, and then were taken mutagenesis by UV and chemical 2 methods. A fusant displaying higher antifreeze capacity, as compared to that of its parents, was obtained by PEGinduced fusion between UVinactivated protoplasts of V3552 EMS and heatinactivated protoplasts of V23 UV through low temperature (0℃) selection. The best fusion condition is gained at 400g/LPEG6000, pH 8.0, for 30min fusion time, at 32℃ fusion temperature. The fusion rate could be as high as 0517%. And 200 fusants were obtained. Through screening with 0℃ low temperature, 15 Volvariella volvacea cold tolerant bacterial strains were obtained. The cold tolerant ability of hypha at 0℃ was improved by 4.5 times. The results proved that the fruit displayed higher antifreeze capacity, as compared with bacterial strains; and the liquefaction phenomenon was obviously postponed. This indicated that strains screened by this method were of further development value.

Key words: Volvariella volvacea, protoplast, ultraviolet mutagenesis, chemical mutagenesis, inactivation, PEG fusion