土壤拮抗微生物对几种草莓病原菌的拮抗作用测试

›› 2007, Vol. 9 ›› Issue (2): 77-81.

土壤拮抗微生物对几种草莓病原菌的拮抗作用测试

赵秀娟[1] 徐文桥[1] 张凤巧[1] 高坡[1] 蒋继志[1] 杜洪忠[1] 王树桐[1,2]

[1]河北农业大学植物保护学院河北省病虫害生物防治工程技术研究中心,河北保定071001 [2]中国热带农业科学院生物技术研究所,热带作物生物技术国家重点实验室,海南海口571101

收稿日期:2007-01-18 修回日期:2007-02-07 出版日期:2007-04-15 发布日期:2009-09-29
作者简介:赵秀娟(1981一)|女|在读硕士；主要从事植物病害生物防治研究。
基金资助:
河北省自然科学基金项目（C200500230）;河北省教委博士科研启动基金项目（971102）;河北农业大学大学生科技创新基金项目（07-ZR-076）.

Bioassay of antagonistic microorganisms from soil against several strawberry disease-causing agents

ZHAO Xiu-juan, XU Wen-qiao, ZHANG Feng-qiao, GAG Po, JIANG Ji-zhi, DU Hong-zhong| WANG Shu-tong

1. Bio-control center of plant diseases and pests of Hebei Province,College of Plant Protection, |Agricultural University of Hebei, Baoding 071001, China;2. State Key Laboratory of Tropical Crop Biotechnology, Institute of Bioscience and Biotechnology Chinese Academy for Tropical Agricultural Sciences, Haikou 571101|China

Received:2007-01-18 Revised:2007-02-07 Online:2007-04-15 Published:2009-09-29

摘要/Abstract

摘要：

测试了分离自60份土样中植物根际微生物对草莓灰霉病菌和草莓重茬病菌的拮抗作用,获得了71个对2种靶标病原菌均表现拮抗作用的菌株。其中44个菌株对Botrytis cinereaPers有拮抗作用;33个菌株对Pestaloti-opsis photiniae有拮抗作用;7个菌株对2种植物病原真菌均有明显的拮抗作用（抑菌带≥5 mm）。用上述7个拮抗菌株及其发酵液对BB.cinerea Pers,P.photiniae,R.solona,Fusarium oxysporu和Verticillium dahliae进一步抑菌测定,结果表明：7个菌株及其发酵液对5种病原真菌均有较强的拮抗作用,具有较广谱的抑菌效果。用拮抗微生物处理后的病原菌厚垣孢子比空白对照病原菌的产生量大,菌丝肿胀变形、细胞质凝聚。

关键词: 拮抗微生物草莓灰霉病草莓重茬病

Abstract:

Seventy microorganism isolates from 60 soil samples were evaluated for the antagonistic activity against Botrytis cinerea Pers, Pestalotiopsis photiniae. Results showed that 44 isolates showed antagonistic activity against B. cinerea Pers and 33 isolates against P. photiniae. The pathogen inhibition effed on both B. cinerea Pers and P. photiniae was achieved by 7 of antagonistic microorganisms in 70 isolates （inhibition zone ≥5 mm on average）. These 7 isolates were tested for the inhibition effect against B. cinerea Pers, P. photiniae, Rhizodonia solani, Fusarium oxysporum and Verticillium dahliae. The results showed that all the 7 isolates had broad-spedrum antifungal adivity on these 5 pathogens. These 5 pathogens developed more chlamydospores, the mycelia were bulging vacuolization and their cytoplasm congregated when the pathogens were treated with the antagonistic microorganisms.

Key words: antagonistic microorganism ;strawberry gray mold , disease in replanting

中图分类号:

S432.44 S432.95

赵秀娟[1] 徐文桥[1] 张凤巧[1] 高坡[1] 蒋继志[1] 杜洪忠[1] 王树桐[1,2]. 土壤拮抗微生物对几种草莓病原菌的拮抗作用测试[J]. , 2007, 9(2): 77-81.

ZHAO Xiu-juan, XU Wen-qiao, ZHANG Feng-qiao, GAG Po, JIANG Ji-zhi, DU Hong-zhong. Bioassay of antagonistic microorganisms from soil against several strawberry disease-causing agents[J]. , 2007, 9(2): 77-81.