关键词: 牛 正常体细胞 端粒酶

Abstract:

Telomerase is a nucleotide-protein complex enzyme mainly composed of the telomerase RNA component （TERC） and the telomerase reverse transcriptase （TERT）, which catalyzes the addition of repeat sequence to telomeric DNA with its RNA component as template. To study the relationship between the different expression of telomerase components and the activity of telomerase, the transcription of two main components of telomerase and the activity status of telomerase in adult bovine tissues were examined. RT-PCR was used to detect the transcription of TERC gene and TERT gene in the heart, kidney, liverand testis of adult cattle and the telomerase activity was determined by Telomeric Repeat Amplification Protocols （TRAP） with silver staining, The TERC mRNA was detected in all of the tissues above, with level increasing. The TERT mRNA was only detected in the testis, not in other tissues. The activity of telomerase was also only detected in the testis. These results demonstrate that testis has high activity of telomerase, which is repressed in most of bovine somatic tissues. The transcription of two components of telomerase is independent, and the TERT is the key component of bovine telomerase.

Key words: cattle, adult somatic cells, telomerase