用立式多层产孢箱同步生产多株生防真菌孢子粉

›› 2007, Vol. 9 ›› Issue (3): 108-111.

用立式多层产孢箱同步生产多株生防真菌孢子粉

陈春冯明光

浙江大学生命科学学院微生物研究所,杭州310058

收稿日期:2007-05-10 出版日期:2007-06-15 发布日期:2009-10-16
作者简介:陈春(1979一)|男|讲师|从事应用微生物研究。E—mail：aspring@zju．edu．cn.
基金资助:
浙江省计划项目（2006C31017）和浙江省博士后科学基金项目（508000-x80601）资助.

Synchronous Production of Conidial Powder of Several Fungal Biocontrol |Agents in an Upright Multi-tray Conidiation Chamber

CHEN Chun, FENG Ming-guang

Institute of Microbiology, College of Life Sciences, Zhejiang University, Hangzhou 310058, China

Received:2007-05-10 Online:2007-06-15 Published:2009-10-16

摘要/Abstract

摘要：

利用自主研制的一台立式多层产孢箱作为固相发酵产孢装置，对2株金龟子绿僵菌（Metarhizium anisopliae）、1株金龟子绿僵菌金龟子变种（Manisopliae var．anisopliae）和2株球孢白僵菌（Beauveria bassiana）同时进行了高纯度孢子粉生产的产能试验。每菌株接种6kg大米基质，等量分置于产孢箱的3层中，于25℃下发酵6d。在5株供试菌中，球孢白僵菌Bb2860菌株的产孢量最高，达到2．2×10^12个孢子/kg大米，表现出优异的工艺性状和商品菌剂开发潜力。

关键词: 生防真菌孢子粉生产立式多层产孢箱

Abstract:

A self-developed upright multi-tray conidiation chamber was used to synchronously produce high-quality conidial powder of fungal biocontrol candidates including two strains of Metarhizium anisopliae, one strain of M. anisopliae var. anisopliae and two strains of Beauveria bassiana. Six kilograms of steamed rice （dry weight） was inoculated with liquid cultures of each fungal strain and then equally allocated to three trays of the chamber for six-day growth and conidiation at 25℃. Among the five strains tested, the strain B. bassiana 2860 had a maximal yield of 2.2×10^12 conidia per kg rice and could be potential for development into commercial formulations against insect pests.

Key words: fungal biocontrol agents, conidial production, upright multi-tray conidiation chamber

中图分类号:

S476.12

陈春冯明光. 用立式多层产孢箱同步生产多株生防真菌孢子粉[J]. , 2007, 9(3): 108-111.

CHEN Chun, FENG Ming-guang . Synchronous Production of Conidial Powder of Several Fungal Biocontrol |Agents in an Upright Multi-tray Conidiation Chamber[J]. , 2007, 9(3): 108-111.