利用复合PCR结合DNA芯片的方法进行快速转基因事件检测

›› 2007, Vol. 9 ›› Issue (4): 101-107.

利用复合PCR结合DNA芯片的方法进行快速转基因事件检测

肖一争[1] 唐咏[1] 宛煜嵩[2] 金芜军[2]

[1]沈阳农业大学,沈阳110161 [2]中国农业科学院生物技术研究所,北京100081

收稿日期:2007-06-05 修回日期:2007-07-16 出版日期:2007-08-15 发布日期:2009-10-16
通讯作者: 金芜军，副研究员，博士，主要从事转基因生物安全研究。Tel：010-68919852：E-mail：jinwujunl218@hotmail．com
作者简介:肖一争|硕士研究生|研究方向为转基因植物检测。
基金资助:
国家“863”计划项目转基因食品安全性评价与转基因农产品检测方法研究（2004AA212222）资助.

Coupling Multiplex PCR with DNA Chips to Rapidly Detect and Identify Genetically Modified Events

XIAO Yi-zheng| TANG Yong| WAN Yu-song, JIN Wu-jun

1. Shenyang Agricultural University, Shenyang 110161 |2. Biotcchnology Research Institute Chinese Academy of Agriculture Sciences, Beijing 100081, China

Received:2007-06-05 Revised:2007-07-16 Online:2007-08-15 Published:2009-10-16

摘要/Abstract

摘要：

以我国批准进口用作加工原料的6种转基因玉米为材料,根据其外源基因、载体构建及插入位点,应用6对特异性引物及35S、NOS引物,设计并优化了复合PCR反应体系,对其进行转基因成分检测.同时尝试利用复合PCR与DNA芯片相结合,对不同转基因玉米混合样品进行检测,探索建立转基因事件快速检测和确认方法.

关键词: 转基因玉米复合PCR DNA芯片

Abstract:

A multiplex PCR system was designed and optimized with 35S and Nos primer and other 6 special primers which are designed according to inserted genes, plasmid constructions and inserted sites to detect 6 transgenic maize events which are permitted to import as product material by China, A primarily designed DNA chip coupled with the multiplex PCR system was also used to detect mixed GM maize samples, We attempted to construct a rapid GM maize events detection and confirmation method.

Key words: GM maize , multiplex PCR , DNA chips

中图分类号:

Q813

肖一争[1] 唐咏[1] 宛煜嵩[2] 金芜军[2]. 利用复合PCR结合DNA芯片的方法进行快速转基因事件检测[J]. , 2007, 9(4): 101-107.

XIAO Yi-zheng| TANG Yong| WAN Yu-song, JIN Wu-jun. Coupling Multiplex PCR with DNA Chips to Rapidly Detect and Identify Genetically Modified Events[J]. , 2007, 9(4): 101-107.