关键词: 有机磷降解酶 烟草 转基因植物

Abstract:

Organophosphorus （OP） compounds which widely used as pesticides in agriculture caused broad-area pollution to environment. In this study, we expressed a bacterial organophosphorus hydrolase （OPH） gene in tobacco plants in order to degrade pesticide residue in the environment by root secreted OPH. The expression vector was constructed. The final expression cassette, containing CaMV 35S promoter, the extensin sequence, ophc2 and nos termination sequence, was designated pBI-E-ophc2. The signal sequence from carrot extensin facilitated OPHC2 extracellular expression. The ophc2 gene then was transformed into tobacco （Nicotiana tabacum cv. Xanthi ） leaf disks by Agrobacterium-mediated transformation. Of 101 transgenie lines, 80 were shown to have the transgene by PCR analysis. 24 of the randomly selected 60 transgenic plants were positive in western blot analysis. The assay of enzyme activity showed that the transgenic plants could secrete OPH into the growth medium and held the bydrolase activity. The degradation of pesticide in the culture medium and the transgenic plants tissue will be reported in the future.

Key words: organophosphorus hydrolase , tobacco , transgenic plant