关键词: 供体细胞 核移植效率 细胞周期 培养代数

Abstract:

The effects of donor cell cycle and different passage on interspecies nuclear transfer were discussed in this research. Bovine oocytes stained by hochest33342 were enucleated as receptor and human fibroblast in different cell cycle and passage were used as donor for nuclear transfer. After electric fusion, these reconstructed embryos were activated by 6-DAMP ＋ ethanol and cultured in SOFaa medium. The fusion and development rates of the treatment inn the 20th passaage cell without starvation （ P 〈 0.01 ） were significantly lower than that in the 20th passage with starvation （ P 〈 0.05 ）, and were significantly lower than that in the 8th passage with starvation and without starvation （P 〈0.01）. There were no significant differences in 8-ccell embryo rates and morula-blastocyst rates in four treatments. The result indicates that starvation treatment may not be the essential factor for lowpassage donor cell and when donor cell is in high passage, starvation treatment can be the important way to improve the efficiency of interspecies nuclear transfer by increasing fusion rate and development rate significantly.

Key words: donor cell , efficiency of nuclear transfer, cell cycle , culture passage numbers