植物无糖组培快繁装置及其环境控制系统的研制

›› 2007, Vol. 9 ›› Issue (4): 79-84.

植物无糖组培快繁装置及其环境控制系统的研制

杨其长刘文科管道平

中国农业科学院农业环境与可持续发展研究所,北京100081

收稿日期:2007-06-26 修回日期:2007-07-10 出版日期:2007-08-15 发布日期:2009-10-16
作者简介:杨其长|研究员|博士|主要研究方向为设施园艺与环境工程。Tel：010-68976901；E-mail：Yangq@cjac．org．cn
基金资助:
科技部仪器升级改造项目（JG-2003-6）和中国农业科学院科研基金项目资助.

Design of Device for Fast Propagation of Plant Sugar-free Tissue and its Environmental Control System

YANG Qi-chang,LIU Wen-ke, GUAN Dao-ping

Institute of Environment and Sustainable Development in Agriculture, Chinese Academy of Agricultural Sciences, Beijing 100081, China

Received:2007-06-26 Revised:2007-07-10 Online:2007-08-15 Published:2009-10-16

摘要/Abstract

摘要：

针对常规植物组培存在的问题,从植物无糖组培实用化角度出发,研制了带有新型CO2施放装置的180 L植物无糖组培容器及其环境控制系统,采用小流量控制、三通阀调节和PWM控制方式,实现了对CO2浓度的精确控制,控制精度达到±50 μmol·mol^-1;采用穴盘覆膜与气体循环吸附相结合的方式实现了对容器内相对湿度的自动控制,控制精度达到±22%.通过圆叶海棠的无糖组培的试验,结果表明,该系统对组培苗的生长环境和生理品质的提高具有显著的促进作用.

关键词: 植物无糖组培培养容器环境控制系统

Abstract:

A 180 L culture vessel for sugar-free tissue culture with new type CO2 releasing pipe and its environment control system were designed, aiming at solving the problems existing in conventional plant tissue culture and from the view point of practicing plant sugar-free micropropagation. By combining thethree techniques of small-flow control, three-way-valve adjustment and PWM control pattern, the CO2 concentration in side of culture vessel was controlled within ±50 μmol · mol^-1 around the target value. The technology of combining membrane tectoria with gas-cycle adsorption was used to realize the auto-control of inner relative humility and the control precision has reached ± 2%. The result of experiment on culturing crabapple plant sugar-free tissue indicates that this system has the function of promoting the growth of tissue culture plantlets and improving their physiological quality.

Key words: plant sugar-free micropropagation, cultural vessel, environmental control system

中图分类号:

Q813.12

杨其长刘文科管道平. 植物无糖组培快繁装置及其环境控制系统的研制[J]. , 2007, 9(4): 79-84.

YANG Qi-chang,LIU Wen-ke, GUAN Dao-ping . Design of Device for Fast Propagation of Plant Sugar-free Tissue and its Environmental Control System[J]. , 2007, 9(4): 79-84.