外源16S rRNA在大肠杆菌中的可替换性研究

›› 2007, Vol. 9 ›› Issue (4): 85-94.

外源16S rRNA在大肠杆菌中的可替换性研究

李晓丹杨瑾曹慧崔中利

南京农业大学生命科学学院微生物学系农业部农业环境微生物工程重点开放实验室,南京210095

收稿日期:2007-05-15 修回日期:2007-06-11 出版日期:2007-08-15 发布日期:2009-10-16

16S rRNA is Exchangeable in Escherichia coli

LI Xiao-dan, YANG Jin, CAO Hui, CUI Zhong-li

Department of Microbiology, Nanjing Agricultural University, Key laboratory of Microbiological |Engineering of Agricultural Environment, Ministry of Agriculture, Nanjing 210095, China

Received:2007-05-15 Revised:2007-06-11 Online:2007-08-15 Published:2009-10-16
About author:Li Xiao—dan|graduate student in molecular biology．Corresponding author：Cui Zhong—li|professor in environmental bioteehaology and molecular ecology．E—mail：ezl@njau．edu．en
Supported by:
National Science Foundation of China （grant No.40371069）, Program for New Century Excellent Talents in University.ACKN0WLEDGMENTS We thank Selywn Quan from the Tufts University for E. coli strain SQ110 used in this work. We also thank Barry L. Wanner of Purdue University for the plasmids used to do the Red recombination. We also thank Dr. Shiming Wang for his discussions and thoughtful reading of the manuscript. This work is partially supported by the National Science Foundation of China （grant No. 40371069）, Program for New Century Excellent Talents in University.

摘要/Abstract

摘要：

16S rRNA对于构建功能性核糖体是十分重要的,人们普遍将其作为原核生物进化中保守的系统发育标志.为了进一步研究16S rRNA的进化,本文将Escherichia coli中最后一个拷贝的16S rRNA基因替换为Bacillus subtilis的16S rRNA 基因,得到了菌株SQ110BSX.菌株SQ110BSX的代时与出发菌株SQ110基本一致,但是SQ110BSX表现出冷敏感性,而且rRNA/蛋白比值为SQ110的148%.实验结果表明,菌株SQ110BSX中的核糖体效率明显下降.由于E.coli和B.subtilis在遗传距离上较远,两者的可替换性证明了16S rRNA的高度保守性.

关键词: 16S rRNA 核糖体效率 rRNA/蛋白比值保守性

Abstract:

16S rRNA plays an important role in the functional construction of ribosomes and is considered to be a conserved phylogenic marker of prokaryotic evolution.To elucidate the evolution of 16S rRNA, we succeeded in replacing the 16S rRNA gene of an E.coli strain with all rrn operons deleted with the 16S rRNA gene of Bacillus subtilis.We found that manipulated strain SQ110BSX showed similar characteristics with the starting E.coli strain SQ110 in terms of generation time.Strain SQ110BSX was cold sensitive, and the rRNA/protein ratio of it increased to 148% of the starting strain.These results indicate that ribosome efficiency has decreased.In view of the phylogenic distance between E.coli and B.subtilis, 16S rRNA was demonstrated to have a highly exchangeable property between these two species of different evolution status.

Key words: 16S rRNA, ribosome efficiency, rRNA/protein ratio, phylogenic conservation

中图分类号:

Q311.4

李晓丹杨瑾曹慧崔中利. 外源16S rRNA在大肠杆菌中的可替换性研究[J]. , 2007, 9(4): 85-94.

LI Xiao-dan, YANG Jin, CAO Hui, CUI Zhong-li . 16S rRNA is Exchangeable in Escherichia coli[J]. , 2007, 9(4): 85-94.