山榛中异戊烯焦磷酸异构酶基因的克隆及分析

›› 2007, Vol. 9 ›› Issue (4): 95-100.

山榛中异戊烯焦磷酸异构酶基因的克隆及分析

汪业春[1] 唐克轩[1,2]

[1]上海交通大学农业与生物学院植物生物技术研究中心,复旦-交大-诺丁汉植物生物技术研发中心,上海200030 [2]复旦大学生命科学学院遗传工程国家重点实验室复旦-交大-诺丁汉植物生物技术研发中心,上海200433

收稿日期:2007-05-11 修回日期:2007-06-23 出版日期:2007-08-15 发布日期:2009-10-16
通讯作者: 唐克轩，教授，博士，主要从事植物分子生物学、植物生物反应器等研究。Tel：021-62932002;-mail：kxtangl@yahoo．com，kxtangl@163．com
作者简介:汪业春|博士研究生|博士期间主要从事药用植物分子生物学研究。
基金资助:
国家“863”计划和上海市科学技术委员会资助.

Molecular Cloning and Characterization of a cDNA Encoding Isopentenyl Diphosphate Isomerase from Hazel （Corylus avellana L. Gasaway）

WANG Ye-chun, TANG Ke-xuan

1. Plant Biotechnology Research Center, School of Agriculture and Biology, Fudan-SJTU-Nottingham Plant Biotechnology R＆D Center, |Shanghai Jiao Tong University, Shanghai 200030 |2. National Key Laboratory of Genetic Engineering, School of Life |Sciences, Fudan-SJTU-Nottingham Plant Biotechnology R＆D Center, Fudan University, Shanghai 200433,China

Received:2007-05-11 Revised:2007-06-23 Online:2007-08-15 Published:2009-10-16

摘要/Abstract

摘要：

采用RACE技术首次报道从产紫杉醇物种山榛中克隆出异戊烯焦磷酸异构酶基因（命名为CgIPI, GenBank登录号为EF553533）,该基因全长为1 196 bp,其中编码区为891 bp,编码一个由296个氨基酸组成的蛋白质.推导出的CgIPI氨基酸序列与其他植物IPI有很高的同源性.Southern blot分析表明,CgIPI基因属于一个小的多基因家族.RT-PCR分析表明,CgIPI基因在根、茎和叶中都表达,且在根中表达最高.该基因的克隆和特征分析将有助于在分子水平上更深入地了解IPI在紫杉醇生物合成中的作用.

关键词: 紫杉醇山榛 RACE 异戊烯焦磷酸异构酶

Abstract:

Here we report for the first time the cloning and characterization of a ftdl-length eDNA encoding isopentenyl diphosphate isomerase （IPP isomerase, EC 5. 3. 3. 2 ）（designated as CglPI, GenBank accession number EF553533 ） from hazel （ Corylus avellana L. Gasaway）, a taxol-producing plant speciess by RACE technique. The full-length eDNA of CglPI was 1 196 bp containing a 891 bp ORF encoding 296 amino acids. Bioinformatic analyses revealed that the deduced CglPI had extensive homology with other plant IPIs. Southern blot analysis indicated that CglPI belonged to a small multi-gene family. Expression analysis revealed that CglPI expression could be detected in roots, stems and leaves, but expressed higher in roots. The cloning and characterization analysis of CglPI gene will enable us to further understand the role of CglPI involved in taxol biosynthetic pathway in hazel at molecular level.

Key words: taxol , Hazel （ Corylus avellana L. Gasaway） , RACE , IPI

中图分类号:

Q78 Q785

汪业春[1] 唐克轩[1,2]. 山榛中异戊烯焦磷酸异构酶基因的克隆及分析[J]. , 2007, 9(4): 95-100.

WANG Ye-chun, TANG Ke-xuan. Molecular Cloning and Characterization of a cDNA Encoding Isopentenyl Diphosphate Isomerase from Hazel （Corylus avellana L. Gasaway）[J]. , 2007, 9(4): 95-100.