瘤胃元基因组BAC文库研究

›› 2007, Vol. 9 ›› Issue (5): 53-57.

瘤胃元基因组BAC文库研究

朱雅新东秀珠黄力董志扬

中国科学院微生物研究所,微生物资源前期开发国家重点实验室,北京100080

收稿日期:2007-06-21 修回日期:2007-08-27 出版日期:2007-10-15 发布日期:2009-10-19
通讯作者: 董志扬，研究员，主要从事纤维素降解酶研究。Tel：010-64807353；E-mail：dongzy@sun．im．ac．cn。黄力，研究员，主要从事l占菌DAN复制及元基因组研究。
作者简介:朱雅新|助理研究员|主要从事环境微生物基因组学研究。E-mail：zhuyx@sun．im．ac．cn
基金资助:
国家“863”计划项目（2004AA214080）资助.

The Application of BAC Library in Metagenomics |Research of Rumen Microbe

ZHU Ya-xin, D0NG Xiu-zhu,HUANG Li, D0NG Zhi-Yang

National Key Laboratory for Pre-Development of Micro-0rganism Resources, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100080,China

Received:2007-06-21 Revised:2007-08-27 Online:2007-10-15 Published:2009-10-19

摘要/Abstract

摘要：

目前，传统的分离培养技术在瘤胃微生物的研究中仍存在一些无法逾越的障碍。元基因组文库（metagenomiclibraries）及相关技术的发展，为探讨环境中未培养微生物以及复杂的微生物间关系创立了一个新的平台。将该技术应用于瘤胃微生物研究有着广阔的前景。本文综述了元基因组文库技术在瘤胃微生物研究中的应用潜力，同时介绍了初步应用BAC文库研究瘤胃微生物工作的一些进展。

关键词: 瘤胃微生物元基因组细菌人工染色体文库

Abstract:

The conventional method depending on culture by separation has encountered un-overstepped obstacle for studying rumen microbes. Yet the development of metagenomic libraries and the related technologies has established a new platform for discussing the relationship between the uncultured microbes and the complicated microbes. The application of this method in rumen microorganisms research should have a broad prospect. This article summarizes the applying potential of metagenomic libraries technology in researches about the rumen BAC Libraries. At the same time, it also introduces some progress in applying this technology for rumen microorganism research.

Key words: rumen, metagenomic libraries, bacterial artificial chromosome library（ BAC Library）

中图分类号:

Q78 Q93-3

朱雅新东秀珠黄力董志扬. 瘤胃元基因组BAC文库研究[J]. , 2007, 9(5): 53-57.

ZHU Ya-xin, D0NG Xiu-zhu,HUANG Li, D0NG Zhi-Yang. The Application of BAC Library in Metagenomics |Research of Rumen Microbe[J]. , 2007, 9(5): 53-57.