油菜花蕾发育及开花过程中ABCD功能基因的表达差异分析

›› 2007, Vol. 9 ›› Issue (5): 80-86.

油菜花蕾发育及开花过程中ABCD功能基因的表达差异分析

胡晓梅祝欣王健美杨毅李旭锋

四川大学生命科学学院,生物资源与生态环境教育部重点实验室,成都610064

收稿日期:2007-08-23 修回日期:2007-08-29 出版日期:2007-10-15 发布日期:2009-10-19
通讯作者: 李旭锋:教授，主要研究方向为植物遗传学。Tel：028—85410957；E—mail：xfli@SCU．edu．an
作者简介:胡晓梅|硕士研究生|研究方向为植物遗传学。Te1：028—85503072；E-mail：xmhu2007@gmail．com
基金资助:
国家自然科学基金项目“甘蓝型油菜雄性不育（polCMS）相关基因之间的蛋白质相互作用研究”（30570986）资助.

Analysis on Differences of ABCD Gene Expression in Bud and Flower Development of Rape （Brassica napus）

HU Xiao-mei, ZHU Xin, WANG Jian-mei, YANG Yi, LI Xu-feng

College of Life Science, Sichuan University, Key Laboratory of Bio-Resources and Eco-Environment of M0E, Chengdu 610064,China

Received:2007-08-23 Revised:2007-08-29 Online:2007-10-15 Published:2009-10-19

摘要/Abstract

摘要：

以油菜处于花蕾发育过程7个生长期的花蕾和花朵为材料，利用优化的半定量RT—PCR技术在转录水平对ABCD模型的6个功能基因，即Apetalal、Apetala2、Apetala3、Pistillata、Agamous、Agamous—likell，进行分析，探讨了油菜花蕾发育及开花过程中6个功能基因表达的差异。结果表明，在油菜花蕾发育及开花过程中ABCD模型中的6个功能基因的表达水平均有变化。研究阐述了ABCD模型在油菜与拟南芥中存在基因表达时期和表达水平相似性和差异，对进一步分析ABCD功能基因在油菜花蕾和花朵中的表达，油菜育种，雄性不育及花型改变的研究具有重要的意义。

关键词: 油菜 ABCD功能基因基因表达半定量RT—PCR

Abstract:

Expression and functions analysis of ABCD genes, which related to the development of floral organ in rape （Brassica napus ）, was carried out by optimized semi-quantitative reverse transcription-polymerase chain reaction （RT-PCR） with the buds and flowers of rape at seven different development stagges as the material, which was the first time to study ABCD model in rape. It indicated that in rape, six of ABCD genes that are Apetalal, Apetala2, Apetala3, Pistillata, Agamous and Agamous-like11, exhibited a variation in the expression level at the different development stages. This paper also expounds the similarity and differences of ABCD model in gene expression and expression level in both Arabidopsis and rape. It is of great significance for further analyzing the expression of ABCD gene in buds and flowers, for rape breeding, and for research on cytoplasmic male sterile and on flower pattern change.

Key words: rape, ABCD genes , gene expression , semi-quantitative RT-PCR

中图分类号:

S565.4 Q78

胡晓梅祝欣王健美杨毅李旭锋. 油菜花蕾发育及开花过程中ABCD功能基因的表达差异分析[J]. , 2007, 9(5): 80-86.

HU Xiao-mei, ZHU Xin, WANG Jian-mei, YANG Yi, LI Xu-feng. Analysis on Differences of ABCD Gene Expression in Bud and Flower Development of Rape （Brassica napus）[J]. , 2007, 9(5): 80-86.