南海底层鱼突额鹦嘴鱼Scarus ovifrons Temminck et Schlegel|1846肠道产酶微生物研究

›› 2007, Vol. 9 ›› Issue (5): 95-100.

南海底层鱼突额鹦嘴鱼Scarus ovifrons Temminck et Schlegel|1846肠道产酶微生物研究

黄光祥[1,2] 周志刚[2] 何夙旭[2] 邵娜[2] 石鹏君[2] 刘玉春[2] 姚斌[2]

[1]华中农业大学水产学院,武汉430074 [2]中国农业科学院饲料研究所,北京100081

收稿日期:2007-08-29 修回日期:2007-09-12 出版日期:2007-10-15 发布日期:2009-10-19
通讯作者: 姚斌，研究员，博士，博士生导师，从事微生物工程研究。Tel：010-68975126：E-mail:yaobin@mail．eaas．net．cn。周志刚，副研究员，博士，硕士生导师．从事水产微生物学研究。
作者简介:黄光祥|硕士研究生
基金资助:
中央级科研院所社会公益研究专项（2005DIB4J038）资助.

Studies on the Intestinal Enzyme-Producing Microflora in |Typical Marine Finfish Scarus ovifrons Temminck et |Schlegel, 1846 at Sublayer of the South China Sea

HUANG |Guang-xiang| ZH0U Zhi-gang, HE Su-xu, SHA0 Na, |SHI Peng-jun, LIU Yu-ehun| YA0 Bin

1. Huazhong Agrieuhural University, Wuhan 430074|2. Feed Resem＇eh Institute, Chinese Academy of Agrieuhural Sciences, Beijing 100081, China

Received:2007-08-29 Revised:2007-09-12 Online:2007-10-15 Published:2009-10-19

摘要/Abstract

摘要：

通过不同培养基对南海底层鱼突额鹦嘴鱼ScantsovifrousTemmincketSchlegel，1846肠道微生物进行分离与16S、18SrDNA鉴定，并构建系统发育树，然后结合选择性培养基进行产蛋白酶、甘露聚糖酶、木聚糖酶、纤维素酶、淀粉酶等微生物的筛选。结果表明，从突额鹦嘴鱼肠道分离纯化出23株微生物，其中14株产酶，以产蛋白酶与淀粉酶为主，部分产纤维素酶、甘露聚糖酶、木聚糖酶；产酶微生物主要为Bacillussp．；分离出3株菌（H-16、J-13与Y-13G）其16S、18SrDNA序列与模式种相似度低于97％，为潜在的新种。研究表明，南海底层鱼突额鹦嘴鱼肠道含有大量产酶微生物。

关键词: 突额鹦嘴鱼Scarus ovifrons Temminck et Schlegel 1846 肠道产酶微生物

Abstract:

The intestinal microflora in Scants ovifrons Temminck et Schlegel, 1846 at suhlayer of the South China Sea were isolated and identified by 16S, 18S rDNA sequences as well as the neighbour-joining phylogenetic trees. And the enzyme-producing microflora such as protein enzyme, mannanase, xylanase, cellulose enzyme, and amylase were screened by selective culture medium. The results showed that 23 isolates of intestinal microflora were identified, in which 14 isolates were the enzyme-producing microflora and mainly excreted protein enzyme and partly excreted cellulose enzyme, mannanase and xylanase; theenzyme-producing microflora were mainly Bacillus sp. ; the isolate of H-16, J-13 or Y-13G from the intestine of Scarus ovifrons Temminck et Schlegel, 1846 might represent new microflora due to the less than 97% similarity after the blast of its 16S or 18S rDNA sequence. The results indicated that rich enzyme-producing microflora existed in the intestine of the marine finfish Scarus ovifrons Temminck et Schlegel, 1846 at sublayer of the South China Sea.

Key words: Scarus ovifrons Temminck et Schlegel, 1846, intestine, enzyme-producing microflora

中图分类号:

Q93-3

黄光祥[1,2] 周志刚[2] 何夙旭[2] 邵娜[2] 石鹏君[2] 刘玉春[2] 姚斌[2]. 南海底层鱼突额鹦嘴鱼Scarus ovifrons Temminck et Schlegel|1846肠道产酶微生物研究[J]. , 2007, 9(5): 95-100.

HUANG Guang-xiang| ZH0U Zhi-gang, HE Su-xu, SHA0 Na, SHI Peng-jun, LIU Yu-ehu. Studies on the Intestinal Enzyme-Producing Microflora in |Typical Marine Finfish Scarus ovifrons Temminck et |Schlegel, 1846 at Sublayer of the South China Sea[J]. , 2007, 9(5): 95-100.