H5亚型血凝素基因的插入对重组LaSota毒株在组织中病毒分布的影响

›› 2008, Vol. 10 ›› Issue (1): 103-107.

H5亚型血凝素基因的插入对重组LaSota毒株在组织中病毒分布的影响

董俊斌[1,2] 王永[1,2] 葛金英[2] 马学恩[1] 步志高[2]

[1]内蒙古农业大学动物科学与医学学院,呼和浩特010018 [2]中国农业科学院哈尔滨兽医研究所兽医生物技术国家重点实验室,哈尔滨150001

收稿日期:2007-12-07 修回日期:2008-01-10 出版日期:2008-02-15 发布日期:2009-07-30
通讯作者: 步志高，研究员，博士，博士生导师，从事人畜共患病研究。Tel：0451-85935062；E-mail：zgb@hvri．ac．cn
作者简介:董俊斌|博士研究生|主要从事分子病理学研究。E-mail：nndxqb@163．com。
基金资助:
973项目（2005CB523200）资助.

The Impact of HA Gene of H5N1 Being Inserted into Recombinate |Lasota Strain on the Distribution of Viral Antigen 2

DONG Jun-bin, WANG Yong, GE Jin-ying, MA Xue-en| BU Zhi-gao

1. College of Animal Science and Medicine, Inner Mongolia Agricultural University, Huhhot 010018 ; |2. National Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, |Chinese Academy of Agricultural Sciences, Harbin 150001, China

Received:2007-12-07 Revised:2008-01-10 Online:2008-02-15 Published:2009-07-30

摘要/Abstract

摘要：

为了研究外源基因H5亚型血凝素基因插入到NDV后对重组LaSota毒株在组织中对NDV病毒分布的影响,用构建的rL-F（M）-FHA和rL-FHA与亲本毒株重组LaSota野毒株（rLaSota）经滴鼻、点眼免疫SPF雏鸡,分别在免疫后1 d、3 d、5 d、10 d、15 d和18d后采集病料（气管、肝脏,肺脏、肾脏、大脑、脾脏、腺胃、十二指肠）,利用免疫组化染色方法检测病毒在机体内的分布。H5亚型血凝素基因的插入对NDV病毒分布、病毒在组织中出现时间有很大影响,而F基因突变株的变化也是影响病毒分布、病毒在组织中出现时间的主要因素。

关键词: H5亚型血凝素基因重组LaSota 病毒分布

Abstract:

In order to study the impact of HA gene of H5N1 on the viral antigen distribution, we inserted foreign gene （HA gene of H5N1 highly pathogenic avian influenza virus） between the M gene and P gene based on the F gene being exchanged rLaSota and rLaSota backbone, which were named as rL-F（M）-FHA and rL-FHA. Vaccination was given by eyedrop and intranasally instillation with rL-F（M）-FHA, rL- FHA and rlaSota to 4-day-old SPF chickens, after 1 d, 3 d, 5 d, 10 d, 15 d or 18 d, we collected the trachea, lung, liver, kidney, brain, spleen, proventriculus, duodenum. The distribution of viral antigen in tissues were detected by using immunohistochemical （ IHC ） staining. The results showed that HA gene of H5N1 being inserted rLaSota and F gene being exchanged had great influence to the distribution of viral antigen.

Key words: HA gene of H5N1 , recombinate LaSota strain, viral antigen distribution

中图分类号:

Q939.47

董俊斌,马学恩,步志高. H5亚型血凝素基因的插入对重组LaSota毒株在组织中病毒分布的影响[J]. , 2008, 10(1): 103-107.

DONG Jun-bin, WANG Yong, GE Jin-ying, MA Xue-en| BU Zhi-gao. The Impact of HA Gene of H5N1 Being Inserted into Recombinate |Lasota Strain on the Distribution of Viral Antigen 2[J]. , 2008, 10(1): 103-107.