丹参丛生芽诱导和植株的高频再生(英文)

›› 2008, Vol. 10 ›› Issue (1): 76-80.

丹参丛生芽诱导和植株的高频再生(英文)

徐妙云[1] 孙启忠[2]

[1]中国农业科学院生物技术研究所,北京100081 [2]中国农业科学院草原研究所,呼和浩特010010

收稿日期:2007-11-01 修回日期:2007-11-30 出版日期:2008-02-15 发布日期:2009-07-30

Buds Induction and High-frequency Plant Regeneration of |Salivia miltiorrhiza Bunge

XU Miao-yun| SUN Qi-zhong

1 Biotechnology Research Institute, Chinese Academy of Agricultural Sciences, Beijing 100081 ; |2. Grassland Research Institute, CAAS, Huhhot 010010, China

Received:2007-11-01 Revised:2007-11-30 Online:2008-02-15 Published:2009-07-30
About author:Xu Miao-yun,assistant researcher,Ph. D,engage in plant biotechnology resesarch. E-mail：xumiaoyun@sohu. com.Corresponding author： Sun Qi-zhong, professor in forage production and meadow improvement. Tel ：0471-4926909 |E-mail ： sunqz@126. com
Supported by:
ACKNOWLEDGEMENTS The authors wish to thank Dr. Liu De-hu from the Bioteehnology Research Institute, Chinese Academy of Agricultural Sciences, and colleagues of the group for their support and help. We thank Dr. Zhang from the Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences for kindly supplying Radix seeds.

摘要/Abstract

摘要：

对丹参直接芽再生系统进行研究,探讨不同基因型、外植体类型（幼茎,下胚轴和叶）和BA、IBA及蔗糖的浓度对其不定芽诱导、伸长和生根的影响。结果表明,来自丹参99-5幼苗的叶外植体芽诱导率最大。诱导芽再生的最佳培养基为MS＋0.1mg·L^-1BA,在该培养基上培养30 d的外植体可获得最多的不定芽。将再生芽转移到MS＋0.1mg·L^-1BA培养基上进行伸长培养,当芽长至3.5 cm时,将其转移至1/2 MS＋1.0mg·L^-1IBA＋10%蔗糖的培养基中诱导生根。

关键词: 丹参不定芽叶外植体再生

Abstract:

A direct induction of adventitious buds and in vitro plantlet regeneration system for Salivia miltiorrhiza Bunge was optimized by studying the influences of genotype, explants type （young stem, petiole and leaf） and different concentration of BA, IBA and surcose. Leaf explants from 99-5 seedlings showed maximum buds induction. The plant growth regulator BA （0. 1 mg·L^-1） was effective in stimulating shoot regeneration from leeaf explants of S. miltiorrhiza. The highest efficiency of bud formation was observed with a 30-day culture in MS containing 1.0 mg ·L^-1 BA. The regenerated buds were transferred to MS medium containing 0.1 mg·L^-1 BA for elongation. When the shoots were about 3.5 cm in height, they were transferred to 1/2-strengh MS medium supplemented with 1.0 mg·L^-1 IBA and 10% sucrose to induce rooting.

Key words: Salivia miltiorrhiza Bunge , adventitious bud , leaf explant , regeneration

中图分类号:

S336

徐妙云,孙启忠. 丹参丛生芽诱导和植株的高频再生(英文)[J]. , 2008, 10(1): 76-80.

XU Miao-yun| SUN Qi-zhong . Buds Induction and High-frequency Plant Regeneration of |Salivia miltiorrhiza Bunge[J]. , 2008, 10(1): 76-80.

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