胡杨叶绿素a/b结合蛋白基因的克隆及序列特性分析

›› 2008, Vol. 10 ›› Issue (4): 63-69.

胡杨叶绿素a/b结合蛋白基因的克隆及序列特性分析

未丽[1,2] 徐秉良[1] 雷江丽[3] 刘水[4] 唐益雄[2] 阴翠翠[2,5] 吴燕民[2]

[1]甘肃农业大学草业学院,兰州730070 [2]中国农业科学院生物技术研究所,北京100081 [3]深圳市园林科学研究所,广东深圳518003 [4]深圳市铁汉园林绿化有限公司,广东深圳518034 [5]西北大学,西安710069

收稿日期:2008-04-08 修回日期:2008-06-23 出版日期:2008-08-15 发布日期:2009-07-29
通讯作者: 吴燕民．研究员，博士，主要从事植物生物反应器和植物基因工程研究。E-mail：Wuym56@163．com
作者简介:未丽|硕士研究生|主要从事植物抗逆分子生物学相关研究。E-mail：weili20002005@yahoo．com．cn。
基金资助:
国家973计划项目（2007CB108902-2）资助.

Cloning and Characterization of a Full-Length Cab Gene |Encoding the Light Harvesting Chlorophyll a/b Binding Protein from Populus euphratica

WEI Li, XU Bing-liang| LEI Jiang-li| LIU Shui, TANG Yi-xiong, |YIN Cui-cui, WU Yan-min

1. Pratacuhural College, Gansu Agricultural University, Lanzhou 730070; |2. Biotechnology Research Institute, Chinese Academy of Agricultural Sciences, Beijing 100081|3. Shenzhen Institute of Landscape Gardening, Guangdong Shenzhen 518003|4. Shenzhen Tiehan Garden Greening Corporation, Guangdong Shenzhen 518034|5. Northwest University, Xi＇an 710069, China

Received:2008-04-08 Revised:2008-06-23 Online:2008-08-15 Published:2009-07-29

摘要/Abstract

摘要：

采用RT-PCR和RACE—PCR技术，首次从胡杨中克隆到捕光叶绿素a／b结合蛋白基因全长cDNA，命名为PeCab（GenBank序列号：EU078170）。序列分析表明：该基因全长为992bp，开放阅读框为792bp，编码263个氨基酸，属于光系统Ⅱ类型ⅠCab基因，与植物Cab家族基因具有较高同源性，并且与双子叶植物的Cab家族基因的亲缘关系更近。半定量RT—PCR分析表明，该基因受光诱导表达，对6-BA、GA3和NAA三种激素的诱导均有应答，但在黑暗和ABA激素处理下，PeCab基因在转录水平上的表达基本没有变化。本研究丰富了Cab家族基因库资源，对揭示胡杨光保护及对各种环境适应性机理的研究奠定了基础。

关键词: 胡杨叶绿素a／b结合蛋白序列特性分析半定量RT-PCR

Abstract:

A full-length eDNA encoding the light harvesting chlorophyll a/b binding protein was cloned firstly by RT-PCR and RACE-PCR from Populus euphratica, designated PeCab （GenBank accession number： EU078170 ）. Sequence analysis showed that the length of PeCab is 992 bp, including a complete open reading frame of 792 bp, encoding a protein of 263 amino acids. It belonged to type Ⅰ of PS Ⅱ. PeCab displayed high sequence identity with previously cloned Cab family genes of plant and was clustered close to Cab family genes of dicotyledon plants. Semiquantitative RT-PCR showed that PeCab was greatly induced by light and was responded to 6-BA,GA3 and NAA. The expression of PeCab did not change at transcriptional stage under darkness and ABA solution treatment. The research is not only to enrich the gene resource of Cab gene family, but also to establish the foundation for discovering the mechanism of light and various conditional adaption of Populus euphratica.

Key words: Populus euphratica , light harvesting chlorophyll a/b binding protein , sequence analysis , semi-quantitative RT-PCR

中图分类号:

Q781

未丽,徐秉良,雷江丽,刘水,唐益雄,阴翠翠,吴燕民. 胡杨叶绿素a/b结合蛋白基因的克隆及序列特性分析[J]. , 2008, 10(4): 63-69.

WEI Li, XU Bing-liang| LEI Jiang-li| LIU Shui, TANG Yi-xiong, YIN Cui-cui, WU. Cloning and Characterization of a Full-Length Cab Gene |Encoding the Light Harvesting Chlorophyll a/b Binding Protein from Populus euphratica[J]. , 2008, 10(4): 63-69.