›› 2008, Vol. 10 ›› Issue (5): 75-78.

• 863课题进展 • 上一篇    下一篇

利血平分子印迹聚合物在饲料检测中的应用

史西志 武爱波 瞿国润 郑素连 张大兵   

  1. 上海交通大学生命科学技术学院,上海200240
  • 收稿日期:2008-06-03 修回日期:2008-09-12 出版日期:2008-10-15 发布日期:2009-07-29
  • 通讯作者: 张大兵,教授,主要从事生物安全、植物分子生物学等研究。E-mail:zhangdb@sju.edu.cn
  • 作者简介:史西志|博士研究生|研究方向为生物安全。
  • 基金资助:

    国家863计划项目(2006AA102438)资助.

Application of Molecularly Imprinted Polymers |for Determination of Reserpine in Feed

SHI Xi-Zhi, WU Ai-bo, Qu Guo-run, ZHENG Su-lian, ZHANG Da-bing   

  1. College of Life Science and Biotechnology, Shanghai Jiao Tong University, Shanghai 200240, China
  • Received:2008-06-03 Revised:2008-09-12 Online:2008-10-15 Published:2009-07-29

摘要:

以获得的利血平(Reserpine,RES)分子印迹聚合物为填料,制备出分子印迹固相萃取小柱,对饲料中的利血平进行富集、纯化;结合高效液相色谱法,建立了基于分子印迹技术的检测饲料中利血平的新方法,灵敏度达0.05μg/g,回收率达80%以上,可应用于实际检测。

关键词: 利血平 分子印迹聚合物 固相萃取

Abstract:

The obtained molecularly imprinted polymers (MIPs) against reserpine (RES) was used as solid phase extraction adsorbents for enrichment and purification of reserpine in feed. Combined with regular HPLC analysis, the new method for reserpine determination based on molecularly imprinted polymers technology was developed, with sensitivity of 0. 05 μg/g, and recovery rate above 80%. This new method can be applied in practice.

Key words: reserpine, molecularly imprinted polymers, solid phase extraction

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