关键词: 鸡 PEPT1基因 Rosetta（DE3） 表达载体

Abstract:

According to the PEPT1 gene sequence in GenBank,specific primer sequences were designed to amplify the PEPT1 epitope fragment gene.The PEPT1 epitope gene was subcloned into expression vector pET-22b(+)to give pET-22b-PEPT1 and expressed in Rosetta(DE3).The expressed product was purified by Ni-NTA chromatography column and identified by SDS-PAGE,Western blot and mass spectrogram.This study was beneficial for further research on producing specific antibody for poultry intestinal PEPT1.

Key words: chicken, PEPT1 gene, Rosetta（DE3）, expression vector;