关键词: 甲基对硫磷水解酶 苍白杆菌 基因克隆 基因表达

Abstract:

Organophosphorus(OP) compounds which are widely used as high efficient and broad-spectrum insecticides caused broad-area pollution to environment.A bactrium with high efficient capability of degrading methyl parathion,identified as Ochrobactrum sp.by 16S rDNA analysis,is isolated from highly OP-polluted soil in this study.The full-length mphxw gene from this bactrium is cloned by PCR.The prokaryotic expression vector is constructed and the expressed products bore normal bioactivity in E.coli.The methyl para...

Key words: methyl parathion hydrolase, Ochrobactrum sp., gene cloning, gene expression;