饲料中4种硝基呋喃类药物的检测方法研究

›› 2008, Vol. 10 ›› Issue (S2): 32-34.

饲料中4种硝基呋喃类药物的检测方法研究

曹莹张文刚黄士新

上海市兽药饲料检测所

出版日期:2008-11-30 发布日期:2009-07-29

Determination of Furazolidone,Furaltadone,Nitrofurazone,Nitrofurantoin in Feed Using HPLC

CAO Ying|ZHANG Wen-gang|HUANG Shi-xin

Shanghai Supervisory Institute of Veterinary Drugs and Feedstuffs|Shanghai 201103|China

Online:2008-11-30 Published:2009-07-29

摘要/Abstract

摘要：

建立了饲料中呋喃唑酮、呋喃它酮、呋喃西林和呋喃妥因4种药物的液相色谱分析方法。液相色谱柱为Waters SunfireTMC18(150 mm×2.1 mm,3.5μm),柱温30℃,流动相为乙腈和0.2%甲酸水溶液,检测波长365 nm,流速0.2 mL/min。此条件下硝基呋喃类药物的线性范围是0.1~10μg/mL,回收率为75%~90%,变异系数为3.24%~5.83%。检测限为0.5 mg/kg,定量限为1.0 mg/kg。

关键词: 饲料硝基呋喃类药物液相色谱

Abstract:

A HPLC method for determination of furazolidone,furaltadone,nitrofurazone and nitrofurantoin in feed was established.The HPLC apparatus was assembled Waters Sunfire C18 column(150 mm×2.1 mm,3.5 μm),detection wavelength was at 365 nm.Aacetonitril and 0.2% formic acid in water was used as mobile phase with a flow rate of 0.2 mL/min.The linearity range of all nitrofran drugs was 0.1~10 μg/mL.The reclaim ratio was 75%~90%.The variation coefficient was 3.74%~5.83%.The limit of detection was 0.5 mg/kg and the lim...

Key words: feed, nitrofuran drugs, HPLC;

中图分类号:

S859.84

曹莹,张文刚,黄士新. 饲料中4种硝基呋喃类药物的检测方法研究 [J]. , 2008, 10(S2): 32-34.

CAO Ying|ZHANG Wen-gang|HUANG Shi-xin. Determination of Furazolidone,Furaltadone,Nitrofurazone,Nitrofurantoin in Feed Using HPLC[J]. , 2008, 10(S2): 32-34.

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