关键词: 高效液相色谱法 维吉尼亚霉素 固相萃取 饲料

Abstract:

A HPLC method to determin the contents of verginamycin in feed was established.The sample was extracted with ethylacetate,purified by silica cartridge and HLB cartridge,and then detected with UV detector or DAD at 210 nm.The concentration of verginamycin and peak area was linear correlation between 2～500 μg/mL,the reclaim ratio was 86.3%～93.3% and RSD was 3.33%～5.37% The detection limit was 0.5 mg/kg and the limit of quantification was 2 mg/kg.The results indicated that the constructed method was accurate,r...

Key words: HPLC, verginamycin, solid-phase extraction, feed;