高效液相色谱法测定配合饲料中氯米芬的含量

›› 2008, Vol. 10 ›› Issue (S2): 48-52.

高效液相色谱法测定配合饲料中氯米芬的含量

战余铭赵明晓朱良智刘华阳李斌

农业部饲料质量监督检验测试中心（济南）烟台市动物防疫监督所

出版日期:2008-11-30 发布日期:2009-07-29

Determination of Clomiphene in Compound Feed Using HPLC

ZHAN Yu-ming|ZHAO Ming-xiao|ZHU Liang-zhi|LIU Hua-yang|LI Bin

1.Feed Quality Supervision and Testing Center（Ji nan）|Ministry of Agriculture|Ji nan 210036|2.Yantai Animal Epidemic Prevention Institute|Shandong Yantai 264000|China

Online:2008-11-30 Published:2009-07-29

摘要/Abstract

摘要：

用甲醇提取配合饲料中的氯米芬,提取液通过MCX小柱进行富集、净化,通过高效液相色谱仪进行检测,利用紫外检测器定量及二极管阵列检测器进行定性。实验表明,顺反式氯米芬分离良好,氯米芬浓度在0.5~25μg/mL范围内与峰面积呈线性相关,r=0.999 9;平均回收率为85%~95%;变异系数≤10%;反式氯米芬检测限为0.3 mg/kg,顺式氯米芬检测限为0.5 mg/kg;常用抗雌激素制剂在该条件下对氯米芬的测定均无干扰。

关键词: 配合饲料氯米芬高效液相色谱仪固相萃取

Abstract:

The clomiphene was extracted from compound feed by methanol.The extract was enriched and purified by MCX-SPE,and then tested by HPLC.Quantitative analysis was carried out by UV-detector and qualitative analysis was by DAD.The result showed that cis-clomiphene and trans-clomiphene could be well seperated,the concentration of clomiphene and peak area was linear correlation between 0.5~25μg/mL,r=0.999 9,the average reclaim ratio was 85%~95%,and coefficient of variation was not exceeding 10%.The LDL of cis-clom...

Key words: compound feed, clomiphene, HPLC, SPE;

中图分类号:

S816.17

战余铭,赵明晓,朱良智,刘华阳,李斌. 高效液相色谱法测定配合饲料中氯米芬的含量[J]. , 2008, 10(S2): 48-52.

ZHAN Yu-ming|ZHAO Ming-xiao|ZHU Liang-zhi|LIU Hua-yang|LI Bin. Determination of Clomiphene in Compound Feed Using HPLC[J]. , 2008, 10(S2): 48-52.

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