高效液相色谱法测定饲料中阿那曲唑

›› 2008, Vol. 10 ›› Issue (S2): 53-56.

高效液相色谱法测定饲料中阿那曲唑

梁萌任爱丽陈淑沂

农业部饲料质量监督检验测试中心（济南）

出版日期:2008-11-30 发布日期:2009-07-29

Determination of Anastrozole in Compound Feed Using HPLC

LIANG Meng|REN Ai-li|Chen Shu-yi

Feed Quality Supervision and Testing Center（Ji nan）|Ministry of Agriculture|Ji nan 210036|China

Online:2008-11-30 Published:2009-07-29

摘要/Abstract

摘要：

称取适量饲料样品,用乙醚和二氯甲烷混合提取液提取后离心,取上层有机相过混合型阳离子交换小柱进行净化处理后,上高效液相色谱仪分析,用紫外检测器和二极管阵列检测器分别在220 nm处进行定量和定性。结果表明,阿那曲唑浓度在0.1~1 000μg/mL范围与峰面积线性良好,回归系数r=0.998 9。回收率为90%~112%,变异系数<5%。阿那曲唑的最低检测限为1μg/mL。4种常用药物在该条件下对阿那曲唑的测定均无干扰。

关键词: 饲料阿那曲唑高效液相色谱

Abstract:

The anastrozole was extracted from compound feed by diethyl ether and dichloromethane.The extract was centrifuged and the supernatant was purified by MCX-SPE,then tested by HPLC.Quantitative analysis was carried out by UV-detector and qualitative analysis was by DAD.The result showed that the concentration of anastrozole and peak area was linear correlation between 0.1~1 000 μg/mL,r=0.998 9.The reclaim rate was 90%~112% with cofficient of variation less than 5%.The LDL of anastrozole was 1 μg/mL.Four medici...

Key words: feed, anastrozole, HPLC;

中图分类号:

S816.17

梁萌,任爱丽,陈淑沂. 高效液相色谱法测定饲料中阿那曲唑 [J]. , 2008, 10(S2): 53-56.

LIANG Meng|REN Ai-li|Chen Shu-yi. Determination of Anastrozole in Compound Feed Using HPLC[J]. , 2008, 10(S2): 53-56.

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