应用两种PCR方法检测饲料中牛、羊源性成分

›› 2008, Vol. 10 ›› Issue (S2): 76-80.

应用两种PCR方法检测饲料中牛、羊源性成分

金凌艳顾欣蔡金华刘雅妮

上海市兽药饲料检测所

出版日期:2008-11-30 发布日期:2009-07-29
基金资助:
上海市科委中小型企业创新资金项目(0639H1N10)资助

Detection of Dovine and Sheep Derived Materials in Feed by Two PCR Methods

JIN Ling-yan|GU Xin|CAI Jin-hua|LIU Ya-ni

Shanghai Supervisory Institute for Veterinary Drugs and Feedstuffs|Shanghai|201103|China

Online:2008-11-30 Published:2009-07-29

摘要/Abstract

摘要：

对普通PCR和实时(realtime)PCR两种方法在饲料中牛、羊源性成分检测中的应用进行了比较。分别设计并合成相应的引物和探针,对饲料中的牛、羊源性成分进行检测。结果表明,用普通PCR最低可以从饲料中检出0.1%的牛、羊源性成分,realtime PCR最低可以从饲料中检出0.01%的牛、羊源性成分。

关键词: 牛、羊源性成分普通PCR 实时PCR

Abstract:

Two polymerase chain reaction methods for the detection of bovine and sheep derived materials in feed were compared.Bovine and sheep derived materials were detected by correponding primers and probes.The result showed that 0.1%bovine and sheep derived materials could be detected by PCR method,and 0.01% bovine and sheep derived materials could be detected by realtime PCR method.

Key words: bovine and sheep derived materials, PCR, realtime PCR;

中图分类号:

S816.17

金凌艳,顾欣,蔡金华,刘雅妮. 应用两种PCR方法检测饲料中牛、羊源性成分 [J]. , 2008, 10(S2): 76-80.

JIN Ling-yan|GU Xin|CAI Jin-hua|LIU Ya-ni. Detection of Dovine and Sheep Derived Materials in Feed by Two PCR Methods[J]. , 2008, 10(S2): 76-80.

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