桦褐孔菌诱变菌株的拮抗试验及酯酶同工酶检测

›› 2009, Vol. 11 ›› Issue (2): 129-132.

桦褐孔菌诱变菌株的拮抗试验及酯酶同工酶检测

冷蕾,黄芳,赵晨,宋爱荣

（青岛农业大学药用真菌研究所, 山东青岛 266109）

收稿日期:2008-12-22 修回日期:2009-01-09 出版日期:2009-04-15 发布日期:2009-07-29
通讯作者: 宋爱荣,主要从事药用真菌有效成分及其发酵提取技术的研究。E-mail：airongsong@163.com
作者简介:冷蕾,硕士研究生,主要从事桦褐孔菌诱变育种及药理作用的研究。E-mail： lenglei@126.com。
基金资助:
山东省科技攻关项目（2007GG2009017）资助。

Tests of Mutant Strains of Inonqqus oblique by Antagonistic
Actions and Esterase Isoenzymes

LENG Lei, HUANG Fang, SONG Ai-rong

(Key Laboratory of Applied Mycology Fungi, Qingdao Agricultural University, Qingdao 266109, China)

Received:2008-12-22 Revised:2009-01-09 Online:2009-04-15 Published:2009-07-29

摘要/Abstract

摘要：

通过60Co-γ辐射诱变桦褐孔菌菌株,对诱变菌株进行拮抗试验,检测其与原始菌株有无拮抗现象；并采

用聚丙烯酰胺凝胶电泳技术对诱变菌株进行酯酶同工酶分析,通过netsys软件对各菌株与原始菌株的差

异性进行了聚类分析。结果表明：诱变菌株均无拮抗现象；但各诱变菌株的酯酶同工酶酶谱有一定差

异。

关键词: 桦褐孔菌；诱变；拮抗；酯酶同工酶

Abstract:

The mutants of Inonqqus oblique treated by radiation of 60Co-γ were tested antagonistic

actions with original strain. Then the esterase isoenzymes from the mutant strains were

studied by using electrophoresis of polyacrylamide gel and cluster analysis were carried

out on the diversities between mutants and original strains by netsys. The results showed

that all mutant strains had no antagonistic actions with original strain 410；the

esterase isoenzymes were different between mutants and original strain.

Key words: Inonqqus obliquus, mutant, antagonism, esterase isoenzyme

中图分类号:

Q939.5

冷蕾,黄芳,赵晨,宋爱荣.
桦褐孔菌诱变菌株的拮抗试验及酯酶同工酶检测[J]. , 2009, 11(2): 129-132.

LENG Lei, HUANG Fang, SONG Ai-rong. Tests of Mutant Strains of Inonqqus oblique by Antagonistic
Actions and Esterase Isoenzymes[J]. , 2009, 11(2): 129-132.