6S rDNA-RFLP法快速鉴定蒙古国传统乳制品中的乳酸菌

›› 2009, Vol. 11 ›› Issue (2): 63-68.

6S rDNA-RFLP法快速鉴定蒙古国传统乳制品中的乳酸菌

艾日登才次克,于洁,杜晓华,李莉,王炜宏,孙天松,张和平

内蒙古农业大学乳品生物技术与工程教育部重点实验室, 呼和浩特 010018

收稿日期:2008-12-07 修回日期:2009-02-20 出版日期:2009-04-15 发布日期:2009-04-15
通讯作者: 张和平,教授,博士生导师,主要从事乳品加工和乳品生物技术研究。Tel：0471-4319940；E-mail：hepingdd@vip.sina.com
作者简介:艾日登才次克,硕士研究生,从事乳品微生物方面的研究。E-mail：caicike123@163.com
基金资助:
国家863计划项目(2006AA10Z345和2007AA10Z353)资助。

Rapid Identification of Lactic Acid Bacteria from Mongolia Home-made Fermented Milk Products Rapidly Identified by16S rDNA-RFLP Method

Airidengcaicike,Yu Jie,DU Xiao-hua,LI Li,WANG Wei-hong,SUN Tian-song,ZHANG He-ping

Key Laboratory of Dairy Biotechnology and Engineering, Ministry of Education, Inner Mongolia Agricultural University|Hohhot 010018, China

Received:2008-12-07 Revised:2009-02-20 Online:2009-04-15 Published:2009-04-15

摘要/Abstract

摘要：

以17株标准菌株为参照，采用限制性片段长度多态性（RFLP）分析和16S rDNA序列分析相结合的技术，对分离自蒙古国5个地区传统乳制品中的55株乳酸菌进行了快速鉴定。结果表明,通过限制性内切酶AluⅠ、HaeⅢ、BsmaⅠ、TspRⅠ和HinfⅠ的指纹图谱分析，将49株乳杆菌和2株片球菌准确鉴定到种，其他4株肠球菌鉴定到属。采用16S rDNA-RFLP方法鉴定乳酸菌具有准确性，可靠性和高效性。

关键词: 乳酸菌；快速鉴定；限制性片段长度多态性(RFLP)；16S rDNA序列分析

Abstract:

55 strains of lactic acid bacteria were isolated from traditional homemade fermented milk products in 5 different regions of Mongolia. All strains were identified on basis of restriction fragment length polymorphism (RFLP) analysis and 16S rDNA sequences compared with 17 standard strains. RFLP analysis used a set of restriction enzymes, AluⅠ, HaeⅢ, BsmaⅠ, TspRⅠand HinfⅠ. The results showed that 49 Lactobacilli strains and 2 Pdeiococcus strains were identified to species level, while the other 4 cocci strains were identified at genus level. 16S rDNARFLP method was of accuracy, reliability and efficiency for identifying lactic acid bacteria.

Key words: lactic acid bacteria, rapid identification, restriction fragment length polymorphism (RFLP), 16S rDNA sequence analysis

中图分类号:

Q93.331

艾日登才次克,于洁,杜晓华,李莉,王炜宏,孙天松,张和平. 6S rDNA-RFLP法快速鉴定蒙古国传统乳制品中的乳酸菌[J]. , 2009, 11(2): 63-68.

Airidengcaicike,Yu Jie,DU Xiao-hua, et al.. Rapid Identification of Lactic Acid Bacteria from Mongolia Home-made Fermented Milk Products Rapidly Identified by16S rDNA-RFLP Method[J]. , 2009, 11(2): 63-68.