草甘膦高抗菌株的分离、鉴定及生理生化特性研究

›› 2009, Vol. 11 ›› Issue (2): 69-72.

草甘膦高抗菌株的分离、鉴定及生理生化特性研究

李海红¹,宛煜嵩²,金芜军²,王英典¹,林敏²

1.北京师范大学生命科学学院, 北京 100875|2.中国农业科学院生物技术研究所, 北京 100081

收稿日期:2009-02-18 修回日期:2009-03-19 出版日期:2009-04-15 发布日期:2009-04-15
通讯作者: 金芜军,副研究员,硕士生导师,主要从事转基因生物安全研究。 Tel:010-82109852;E-mail:Jinwujun1218@yahoo.com.cn
作者简介:李海红,硕士研究生,研究方向为环境微生物基因工程。Tel:010-82109852|E-mail:lihaihong198310@163.com
基金资助:
国家863计划项目（2007AA021304）资助。

Isolation, Identification and Characterization of a Glyphosate-tolerant Bacterial Strain

LI Hai-hong¹, WAN Yu-song²,JIN Wu-jun²,WNAG Ying-dian¹,LIN Min²

1.College of Life Sciences,Beijing Normal University, Beijing 100875|2.Biotechnology Research Institute, Chinses Academy of Agricultural Sciences, Beijing 100081, China

Received:2009-02-18 Revised:2009-03-19 Online:2009-04-15 Published:2009-04-15

摘要/Abstract

摘要：

从采自新疆的土壤中分离到一株草甘膦抗性菌株P23，该菌株能够耐受200 mmol/L的草甘膦,可在最高含350 mmol/L草甘膦的培养基中生长。在草甘膦浓度为100～200 mmol/L之间时，菌株生长迅速，最适pH为7.0,最适生长温度为37℃，具有氨苄青霉素、卡那霉素抗性。用16S rDNA通用引物，经PCR扩增、测序得到P23的16S rDNA序列，该序列在GenBank的登录号为FJ374126。在NCBI中经BLAST后发现与苍白杆菌属(Ochrobactrum)的16S rDNA序列相似性达到99%。结合各项生理生化指标鉴定结果将菌株命名为人苍白杆菌P23（Ochrobactrum anthropi P23）。

关键词: 草甘膦;筛选;鉴定;16S rDNA;人苍白杆菌

Abstract:

A bacterial strain P23, which can tolerate 200 mmol/L glyphosate and grow in highest concentration of glyphosate 350 mmol/ L in the medium, was isolated from soil of Xinjiang. The cell grows well in the medium with 100~200 mmol/L glyphosate, the optimal pH is 7.0 and the optimal growth temperature is 37℃. It has resistance to ampicillin, kanamycin.Using the universal primers, the 16S rDNA of P23 was amplified by PCR and sequenced. Compared with the published nucleotide sequence of 16S rDNA in NCBI, the 16S rDNA of P23 showed 99% homology with Ochrobactrum sp. According to the morphological, physiological and biochemical characteristics, the strain is identified as Ochrobactrum anthropi P23.

Key words: glyphosate, screen, identification, 16S rDNA, Ochrobactrum anthropi

中图分类号:

Q939

李海红,宛煜嵩,金芜军,王英典,林敏. 草甘膦高抗菌株的分离、鉴定及生理生化特性研究[J]. , 2009, 11(2): 69-72.

LI Hai-hong, WAN Yu-song,JIN Wu-jun,WNAG Ying-dian,LIN Min. Isolation, Identification and Characterization of a Glyphosate-tolerant Bacterial Strain[J]. , 2009, 11(2): 69-72.