亚硝酸还原酶高产菌株的筛选及发酵条件优化

›› 2009, Vol. 11 ›› Issue (3): 81-87.

亚硝酸还原酶高产菌株的筛选及发酵条件优化

郑怀忠^1,2,陈发河¹,李淑燕²,孙君社²,刘萍²

1.集美大学生物工程学院, 福建厦门 361021|2.中国农业大学食品科学与营养工程学院, 北京 100083

收稿日期:2009-03-10 修回日期:2009-04-16 出版日期:2009-06-15 发布日期:2009-06-15
通讯作者: 刘萍，副教授，博士，主要从事食品生物技术、生物制药研究。Tel:010-62737131;E-mail:liuping@cau.edu.cn
作者简介:郑怀忠,硕士研究生,研究方向为食品生物技术。
基金资助:
国家863计划项目（2008AA10Z306）资助。

Screening of High Nitrite Reductase Producing Strains and the Optimization of Fermentation

ZHENG Huai-zhong ^1,2, CHEN Fa-he ¹, LI Shu-yan ², SUN Jun-she ², LIU Ping ²

1.College of Bio-engineering, Jimei University, Fujian Xiamen 361021|2.College of Food Science and Nutritional Engineering, China Agricultural University, Beijing 100083, China

Received:2009-03-10 Revised:2009-04-16 Online:2009-06-15 Published:2009-06-15

摘要/Abstract

摘要：

通过紫外线对巨大芽孢杆菌MPF-906进行了诱变处理，提高了产酶能力，变异株Z85酶活达到28.76 U/mL。进一步对其发酵条件进行研究，优化了培养基和培养条件。优化的培养基为：葡萄糖1.5%，牛肉膏0.2%，硫酸铵0.1%，K₂HPO₄ 0.135%，KH₂PO₄ 0.07%，NaCl 0.1%；无机盐溶液10 mL/L（即MgSO₄·7H₂O 0.04%、MnSO₄·H₂O 0.001%、CaCl₂·2H₂O 0.002%）。发酵条件为：摇床转速160 rpm，接种量4%，初始pH 6.5，30 ℃发酵20 h。在此最优化条件下，亚硝酸还原酶的酶活达到45.94 U/mL。

关键词: 亚硝酸还原酶；紫外诱变；发酵；条件优化

Abstract:

The nitrite reductase production strain B.megaterium MPF-906 was treated with UV. The high nitrite reductase producing mutant Z85 was obtained and its nitrite reductase activity was 28.76 U/mL. The effects of compositions of culture medium and cultural conditions on nitrite reductase production were investigated. The optimal culture media are: glucose 1.5%, beef extract 0.2%, (NH4)₂SO₄ 0.1%, K₂HPO₄ 0.135%, KH₂PO4 0.07%, NaCl 0.1%, MgSO₄·7H2O 0.04%, MnSO4·H₂O 0.001%, CaCl₂·2H₂O 0.002%. The fermentation conditions are 30℃ for 20 h, 160 rpm, initial pH 6.5 and 4% of inoculation. Under this optimized conditions, the nitrite reductase activity reached 45.94 U/mL.

Key words: nitrite reductase, UV, fermentation, condition optimization

中图分类号:

TQ920.6⁺1

郑怀忠,陈发河,李淑燕,孙君社,刘萍. 亚硝酸还原酶高产菌株的筛选及发酵条件优化[J]. , 2009, 11(3): 81-87.

ZHENG Huai-zhong, CHEN Fa-he, LI Shu-yan, SUN Jun-she, LIU Ping. Screening of High Nitrite Reductase Producing Strains and the Optimization of Fermentation[J]. , 2009, 11(3): 81-87.