蒙古口蘑多糖提取工艺的研究

›› 2009, Vol. 11 ›› Issue (4): 129-132.

蒙古口蘑多糖提取工艺的研究

渠志臻¹,姚庆智¹,闫伟²

1.内蒙古农业大学生物工程学院, 呼和浩特 010018;2.内蒙古农业大学林学院, 呼和浩特 010018

收稿日期:2008-11-02 修回日期:2009-06-04 出版日期:2009-08-15 发布日期:2009-07-24
通讯作者: 姚庆智,副教授,从事应用与环境微生物学的研究。E-mail：yaoqingzhi@163.com
作者简介:渠志臻,硕士研究生,从事资源微生物学研究。E-mail：realququ@163.com
基金资助:
内蒙古农业大学博士基金资助项目（k13625）资助。

Optimization of Polysaccharide Extraction from Tricholoma Mongolicum Imai.

QU Zhi-zhen ¹, YAO Qing-zhi ¹, YAN Wei ²

1.College of Bioengineering, Inner Mongolia Agricultural University, Hohhot 010018;2.College of Forestry, Inner Mongolia Agricultural University, Hohhot 010018, China

Received:2008-11-02 Revised:2009-06-04 Online:2009-08-15 Published:2009-07-24

摘要/Abstract

摘要：

以蒙古口蘑（Tricholoma mongolicum Imai.）野生子实体和人工液体培养的菌丝体为研究对象，采用常规热水浸提法，对影响多糖提取得率的因素以正交试验的方法进行优化。结果表明,蒙古口蘑子实体多糖提取的最佳工艺为:提取时间4 h，提取4次，提取温度70℃，醇析浓度80%，料液比1∶10,经此工艺子实体的多糖得率为5.78%;菌丝体多糖提取最优工艺为：提取时间3 h, 提取3次，提取温度90℃，醇析浓度80%，料液比1∶10,经此工艺菌丝体的多糖得率为5.93%。

关键词: 蒙古口蘑；多糖；提取工艺

Abstract:

Orthogonal experiments were applied to optimize the polysaccharide extraction from fruiting bodies and mycelium of Tricholoma mongolicum Imai. respectively. The optimal extraction parameters for fruiting bodies were predicted as followed: extraction temperature was 70℃, the ratio of raw material and water was 1∶10, extraction time was 4 hours, extraction was done for 4 times and by 80% (V/V) of ethanol for polysaccharide precipitation. With this technics, the yield of polysaccharides was 5.78%. The optimum extraction conditions for mycelium were extracted at temperature of 90℃, with ratio between raw material and water as 1∶10. The extract would last for 3 hours, and be made for 3 times and by 80% ethanol for polysaccharide precipitation. With this technics, the yield of polysaccharides was 5.93%.

Key words: Tricholoma mongolicum Imai., polysaccharide, extraction technics

中图分类号:

S646.9

渠志臻,姚庆智,闫伟. 蒙古口蘑多糖提取工艺的研究[J]. , 2009, 11(4): 129-132.

QU Zhi-zhen, YAO Qing-zhi, YAN Wei. Optimization of Polysaccharide Extraction from Tricholoma Mongolicum Imai.[J]. , 2009, 11(4): 129-132.