拟南芥miRNA及其靶序列配对特征分析

›› 2009, Vol. 11 ›› Issue (4): 53-57.

拟南芥miRNA及其靶序列配对特征分析

刘冲¹,2,孙杰¹,范云六²,王磊²

1.石河子大学农学院, 绿洲生态实验室, 新疆 832003;2.中国农业科学院生物技术研究所,国家农作物基因资源与基因改良重大科学工程, 北京 100081

收稿日期:2009-03-31 修回日期:2009-06-22 出版日期:2009-08-15 发布日期:2009-07-24
通讯作者: 王磊，研究员，博士，从事基因沉默的研究。E-mail:wanglei@caas.net.cn
作者简介:刘冲,硕士研究生,研究方向为植物代谢工程。E-mail:liu_chong02@163.com
基金资助:
国家863计划项目（2007AA10Z147）；绿洲生态农业重点实验室开放课题发展基金（200704）资助。

Characterization of Arabidopsis microRNAs |and Matching to its Targets

LIU Chong^1,2, SUN Jie ¹, FAN Yun-liu ², WANG Lei ²

1.College of Agriculture, Key Laboratory of Oasis Ecology Agriculture, Shihezi University, Xinjiang Shihezi 832003;2.Biotechnology Research Institute, |National Key Facility of Crop Gene Resources and Genetic Improvement, Chinese Academy of Agricultural Sciences, Beijing 100081, China

Received:2009-03-31 Revised:2009-06-22 Online:2009-08-15 Published:2009-07-24

摘要/Abstract

摘要：

microRNAs(miRNAs)是真核生物中一类长度约为22 nt的非编码小分子RNA，miRNA与AGO等蛋白形成RISC沉默复合体，通过剪切或翻译抑制对靶基因起负调控作用。对拟南芥miRNA序列及其配对的靶序列间的特征进行了统计分析，结果表明miRNA序列5′端富含A、U，第1、第19碱基位对U、C具有较强的倾向性；miRNA与靶序列间常有1~4个碱基错配，错配碱基常出现在第1，第2和第21位，而第3~第6，第9~第10，第16~第17碱基配对较为保守，为人工合成miRNA的设计及miRNA靶基因的预测以提供了依据。

关键词: 拟南芥;基因沉默;RNAi;微小RNA;人工小RNA

Abstract:

MicroRNAs (miRNAs) are a class of endogenous non-coding single-stranded RNA with about 22 nucleotides length. MicroRNAs function as sequencespecific negative regulators in post-transcriptional gene silencing by base pairing with target mRNAs, which lead to mRNA cleavage or translational repression. We examined the sequence characteristics of all known Arabidopsis miRNAs and the basepairing feature between miRNAs and their targets. The miRNA sequences displayed higher A and U content at the 5′ end, and higher U content at position 1, and higher C content around position 19. There often exist one to four mismatching between miRNAs and their targets, which showed that mismatches occurred frequently at position 1, 2 or 21, but hardly occurred at position 3~6, 9~10, 16~17. The data is potentially applicable to the design of artificial microRNA and prediction of microRNAs targets.

Key words: Arabidopsis, gene silencing, RNAi, microRNA, artificial microRNA(amiRNA)

中图分类号:

Q522+.6

刘冲,孙杰,范云六,王磊. 拟南芥miRNA及其靶序列配对特征分析[J]. , 2009, 11(4): 53-57.

LIU Chong, SUN Jie, FAN Yun-liu, WANG Lei . Characterization of Arabidopsis microRNAs |and Matching to its Targets[J]. , 2009, 11(4): 53-57.