黑嘴病及生殖对虾夷马粪海胆五种生理生化指标的影响

›› 2009, Vol. 11 ›› Issue (4): 77-82.

黑嘴病及生殖对虾夷马粪海胆五种生理生化指标的影响

张勃,丁君,王媛,董旭,常亚青

大连水产学院|农业部海洋水产增养殖学重点开放实验室,辽宁大连 116023

收稿日期:2009-04-04 修回日期:2009-06-05 出版日期:2009-08-15 发布日期:2009-07-24
通讯作者: 常亚青,教授,博士生导师,主要从事水产动物遗传育种生物技术方面的研究。Tel:0411-84762695;E-mail:yqchang@dlfu.edu.cn
作者简介:张勃,硕士研究生,从事水产动物遗传与育种方面的研究。E-mail:zhangfish_2003@126.com
基金资助:
国家863计划项目(2006AA10A411);辽宁省科技攻关计划项目(2007203004);辽宁省创新团队项目(2007t017); “百千万人才工程”项目（2008921059）资助。

Effects of Black Mouth Disease and Reproduction on Five Physiological and Biochemical Parameters of Sea Urchins (Strongylocentrotus intermedius)

ZHANG Bo, DING Jun, WANG Yuan, DONG Xu, CHANG Ya-qing

Dalian Fisheries College, Key Laboratory of Mariculture, Ministry of Agriculture, Liaoning Dalian 116023, China

Received:2009-04-04 Revised:2009-06-05 Online:2009-08-15 Published:2009-07-24

摘要/Abstract

摘要：

对虾夷马粪海胆(Strongylocentrotus intermedius) 患“黑嘴病”及生殖后体腔液中5种生理生化指标进行了检测，结果表明病理情况下的虾夷马粪海胆过氧化氢含量和丙二醛含量明显高于对照组，而过氧化氢酶活性、超氧化物歧化酶活性和总抗氧化能力明显低于对照组，其中，过氧化氢含量、总抗氧化能力和超氧化物歧化酶活性差异显著（P<0.05），丙二醛含量、过氧化氢酶活性差异极显著（P<0.01）。生殖后除过氧化氢含量外其他4种生理生化指标均降低，其中总抗氧化能力和丙二醛含量差异显著（P<0.05），过氧化氢酶和超氧化物歧化酶活性差异极显著（P<0.01）。研究表明以海胆体腔液中丙二醛含量和过氧化氢酶活性作为海胆黑嘴病发生的监测指标是可行的。

关键词: 虾夷马粪海胆；黑嘴病；生殖；指标

Abstract:

Five physiological and biochemical parameters were studied in sea urchins (Strongylocentrotus intermedius) suffering from black mouth disease and after breeding. The results showed that under pathological status, the H₂O₂content and malondialdehyde (MDA) content were significantly higher than those of control sea urchins, while catalase (CAT) activity, superoxide dismutase (SOD) activity and total antioxidant capacity (T-AOC) were notablely lower than those in control ones. Among these parameters, the H₂O₂ content, T-AOC and SOD activity were significantly different (P<0.05) between control and sick groups, while the difference of MDA content and CAT activity were extremely significant (P<0.01) between them. After breeding, the H₂O₂content remained while the other four types of immune parameters decreased, among which the T-AOC and MDA content were significantly different (P<0.05), while the difference of CAT activity and SOD activity between after and before breeding were extremely significant (P<0.01). The results suggest that the content of MDA and CAT of coelomic liquid can be used as monitoring indicators of black mouth disease in sea urchins.

Key words: Strongylocentrotus intermedius, black mouth disease, breeding, parameters

中图分类号:

S947

张勃,丁君,王媛,董旭,常亚青. 黑嘴病及生殖对虾夷马粪海胆五种生理生化指标的影响[J]. , 2009, 11(4): 77-82.

ZHANG Bo, DING Jun, WANG Yuan, DONG Xu, CHANG Ya-qing. Effects of Black Mouth Disease and Reproduction on Five Physiological and Biochemical Parameters of Sea Urchins (Strongylocentrotus intermedius)[J]. , 2009, 11(4): 77-82.