基于酶催化沉积放大的检测芜菁花叶病毒的生物传感技术

›› 2009, Vol. 11 ›› Issue (5): 125-129.

基于酶催化沉积放大的检测芜菁花叶病毒的生物传感技术

蒋小平1,李明福2,陈洪俊2,吴会旺1,谭钟扬1,蒋健晖1,沈国励1,俞汝勤1

（1.湖南大学生命科学与技术研究院, 长沙 410082|2.中国检验检疫科学研究院, 北京 100022）

收稿日期:2009-06-05 修回日期:2009-09-04 出版日期:2009-10-15 发布日期:2009-10-15
通讯作者: 谭钟扬,教授,从事植物病毒分子生物学和生物传感技术研究。E-mail:hunandaxuetzy@163.com
作者简介:蒋小平,硕士研究生,从事植物病毒分子生物学研究。
基金资助:
“十一五”国家科技支撑计划项目(2006BAD08A13)资助。

Biological Sensing Technology Based on Enzyme-catalyzed Depositing Enlargement Used for Detection of Turnip Mosaic Virus

JIANG Xiao-ping1, LI Ming-fu2, CHEN Hong-jun2, WU Hui-wang1, |TAN Zhong-yang1, JIANG Jian-hui1, SHEN Guo-li1, YU Ru-qin1

(1.Institute of Life Science and Biotechnology, Hunan University, Changsha 410082|2.Chinese Academy of Inspection and Quarantine, Beijing 100022, China)

Received:2009-06-05 Revised:2009-09-04 Online:2009-10-15 Published:2009-10-15

摘要/Abstract

摘要：

提出了一种基于酶催化沉积放大检测芜菁花叶病毒(Turnip mosaic virus, TuMV)的生物传感技术。该方法先通过夹心免疫反应,将碱性磷酸酯酶标记的芜菁花叶病毒抗体固定到电极表面,然后通过碱性磷酸酯酶催化还原银离子在电极表面形成不溶性沉积物,从而放大电化学检测信号。考察了抗体的用量和沉积时间对免疫分析的影响,结果显示传感器信号响应与芜菁花叶病毒原液的浓度在10~1 000倍稀释范围内呈良好的线性关系,检出限达到500 000倍芜菁花叶病毒原液稀释浓度。

关键词: 芜菁花叶病毒；免疫传感器；碱性磷酸酯酶；阳极溶出伏安法

Abstract:

A novel bio-sensing technology based on enzyme-catalyzed depositing enlargement was proposed to detect Turnip mosaic virus (TuMV). By using this method, the alkaline phosphatase-labeled antibody (TuMV antibody) was firstly immobilized on the electrode surface through sandwich immunity reaction, then the infusible deposition was formed on the electrode surface by using alkaline phosphatase catalyze ascorbic acid to reduce silver ions. Thus the electrochemical signal of the electrode was enlarged greatly. The effects of experimental conditions, such as the concentration of antibody and the depositing time on the performances of immunoassay were studied. The result showed a fine linear relationship between reduction wave peak current and the concentration of TuMV from 10 to 1 000-fold dilution of TuMV primary solution with the detection limit of 500 000-fold dilution of TuMV primary solution.

Key words: TuMV, immunosensor, alkaline phosphatase, anodic stripping voltammetry

中图分类号:

S41-30

蒋小平1,李明福2,陈洪俊2,吴会旺1,谭钟扬1,. 基于酶催化沉积放大的检测芜菁花叶病毒的生物传感技术[J]. , 2009, 11(5): 125-129.

JIANG Xiao-ping1, LI Ming-fu2, CHEN Hong-jun2, WU Hui-wang1, . Biological Sensing Technology Based on Enzyme-catalyzed Depositing Enlargement Used for Detection of Turnip Mosaic Virus[J]. , 2009, 11(5): 125-129.