白细胞介素6及其受体在绵羊卵母细胞成熟过程中的表达

›› 2009, Vol. 11 ›› Issue (5): 71-76.

白细胞介素6及其受体在绵羊卵母细胞成熟过程中的表达

杨美玲,赵锡安,刘东军,仓明

（内蒙古大学哺乳动物生殖生物学及生物技术教育部重点实验室, 呼和浩特 010021）

收稿日期:2009-05-13 修回日期:2009-08-12 出版日期:2009-10-15 发布日期:2009-10-15
通讯作者: 仓明,副研究员,博士,主要从事哺乳动物生殖生物学及生物技术领域研究。Tel:0471-4992443;E-mail:cangming1970@yahoo.com.cn
作者简介:杨美玲,硕士研究生,研究方向为动物生殖生物学。
基金资助:
国家863计划项目（2008AA101007）;国家自然科学基金项目(30760168)资助。

Expression of Interleukin-6 and Its Receptor mRNA in Sheep Oocytes Maturation Process

YANG Mei-ling, ZHAO Xi-an, LIU Dong-jun, CANG Ming

(Key Laboratory of Mammal Reproduction Biology and Biotechnology, Ministry of Education, Inner Mongolia University, Hohhot 010021, China)

Received:2009-05-13 Revised:2009-08-12 Online:2009-10-15 Published:2009-10-15

摘要/Abstract

摘要：

采用实时定量PCR技术检测了不同成熟培养时间绵羊卵母细胞和卵丘细胞白细胞介素6基因（interleukin-6 gene, IL-6）及其受体基因（interleukin-6 receptor gene, IL-6R）mRNA的相对表达量。结果显示,在体外成熟培养过程中,卵母细胞和卵丘细胞中持续表达IL-6及其受体IL-6R mRNA,培养4~8 h,其相对表达量呈现显著升高,随后则降低；在16~20 h时卵母细胞中IL-6 mRNA表达重新升高；相应的卵丘细胞在12~16h时IL-6R mRNA表达也升高。绵羊卵母细胞和卵丘细胞中IL-6和IL-6R mRNA的持续表达以及动力学变化提示了白细胞介素6在卵母细胞成熟过程中具有潜在调控作用。

关键词: 白细胞介素6;卵母细胞;体外成熟;实时定量PCR

Abstract:

The relative expressions of the Interleukin-6 (IL-6) and its receptor (IL-6R) mRNAs in the sheep ovine oocytes maturation stage was detected by the relative real-time RT-PCR. The results demonstrated that the Interleukin-6 and its receptor mRNAs were expressed continuously during ovine oocytes maturation in vitro, with significant high expression in the maturation at 4~8 h, then decreased dramatically. However IL-6 in the oocytes increased again at 16~20 h, correspondingly IL-6R in the cumulus cells increased at 12~16 h. Dynamic changes and the persistent presence of IL-6 signaling system within oocytes and cumulus cells open up the possibility of a potential regulatory role played by cytokine in ovine oocytes maturation.

Key words: IL-6, oocyte, in vitro maturation, real-time PCR

中图分类号:

S814.6

杨美玲,赵锡安,刘东军,仓明. 白细胞介素6及其受体在绵羊卵母细胞成熟过程中的表达[J]. , 2009, 11(5): 71-76.

YANG Mei-ling, ZHAO Xi-an, LIU Dong-jun, CANG Ming. Expression of Interleukin-6 and Its Receptor mRNA in Sheep Oocytes Maturation Process[J]. , 2009, 11(5): 71-76.

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