饲料中三聚氰胺的ELISA检测方法的探讨

›› 2009, Vol. 11 ›› Issue (S1): 50-51.

• 药物残留与致病菌检测 • 上一篇下一篇

饲料中三聚氰胺的ELISA检测方法的探讨

郭洁1,李宏1,贾青1,杨汉卿1,陈瑛2,冯西辉1

(1.农业部饲料质量监督检验测试中心, 西安 |710016|2.铜川市印台区畜牧站, 陕西铜川 727000)

收稿日期:2009-10-15 修回日期:2009-11-20 出版日期:2009-12-16 发布日期:2009-12-15
作者简介:郭洁,助理畜牧师,主要从事饲料产品质量安全检验工作。Tel:029-86253278

Discussion on Melamine Determination in Feed by ELISA Method

GUO Jie1, LI Hong1, JIA Qing1, YANG Han-qing1, CHEN Ying2, FENG Xi-hui1

(1.Feed Quality Supervision and Testing Center, Ministry of Agriculture, Xian 710016|2.Tongchuan City Yintai District Animal Husbandry Station, Shaanxi Tongchuan 727000, China)

Received:2009-10-15 Revised:2009-11-20 Online:2009-12-16 Published:2009-12-15

摘要/Abstract

摘要：

试验利用BRAXIS试剂盒和自制（经气相色谱质谱法确证不含三聚氰胺）三聚氰胺添加量为2 mg/kg的配合料和浓缩料作为阳性对照样品,对100批次饲料产品的进行了三聚氰胺筛选,并将两种方法的筛选结果按照NY/T1372-2007中的气相色谱质谱法进行了确证和比较。试验结果表明：利用三聚氰胺添加量为2 mg/kg配合料和浓缩料为阳性对照样品,对样品的筛选结果,要比使用BRAXIS试剂盒的内制曲线筛选的结果,产生的假阳性少,准确率相对要高。

关键词: 三聚氰胺;检测;ELISA方法

Abstract:

The research made use of BRAXIS kit and self-made compound and concentrated feed(via gas chromatography-mass spectrometry confirmed melamine-free) that melamine adding amount is 2 mg/kg as a positive control sample, screened melamine in 100 batches of pig and chicken feed products. Screening results of two methods in accordance were confirmed and compared with NY/T1372-2007 gas chromatography-mass spectrometry. The results showed that: the use of compound and concentrated feed that melamine adding amount is 2mg/kg as a positive control sample, the results were in fewer false-positive rate and higher accuracy than the use of BRAXIS Kit for the results of screening within the system of curves.

Key words: melamine, determination, ELISA method

中图分类号:

S816.17

郭洁1,李宏1,贾青1,杨汉卿1,陈瑛2,冯西辉1. 饲料中三聚氰胺的ELISA检测方法的探讨[J]. , 2009, 11(S1): 50-51.

GUO Jie1, LI Hong1, JIA Qing1, YANG Han-qing1, CHEN Ying2, FENG Xi-hui1. Discussion on Melamine Determination in Feed by ELISA Method[J]. , 2009, 11(S1): 50-51.

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饲料中三聚氰胺的ELISA检测方法的探讨

Discussion on Melamine Determination in Feed by ELISA Method

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