高效液相色谱法测定灵芝多糖含量

›› 2009, Vol. 11 ›› Issue (S1): 65-67.

高效液相色谱法测定灵芝多糖含量

韩振泰,赵玉娟,刘惠文,李如华

(中国林科院森林生态环境与保护研究所, 国家林业局森林生态环境重点实验室, 北京 100091)

收稿日期:2009-08-15 修回日期:2009-10-28 出版日期:2009-12-16 发布日期:2009-12-15
作者简介:韩振泰,高级实验师,从事液相色谱分析研究。
基金资助:
中央级公益性科研院所基本科研业务费专项(CAFYRIF200708)资助。

Determination of Ganoderma Lucidum Polysaccharide by Reversed-phase High Performance Liquid Chromatography

HAN Zhen-tai, ZHAO Yu-juan, LIU Hui-wen, LI Ru-hua

(Key Laboratory of Forest Ecological Environment of Ministry of Forestry|Research Institute of Forestry Ecology, Environment and Protection, Chinese Academy of Forestry, Beijing 100091, China)

Received:2009-08-15 Revised:2009-10-28 Online:2009-12-16 Published:2009-12-15

摘要/Abstract

摘要：

建立了高效液相色谱法测定灵芝多糖含量的方法,采用糖专用分析柱,以104分子量的葡聚糖为标准,流动相为水,流速0.6 mL/min,检测器为示差检测器,柱温为70℃,检测限为 0.03 g/L,线性方程为Y=109 779.6X-903.3相关系数r=0.999 9,方法回收率为98%～104%,相对标准偏差为RSD%=0.12(n=6)。该方法简易、稳定,适合灵芝多糖的测定。

关键词: 高效液相色谱;示差检测器;灵芝;多糖

Abstract:

A reversed-phase high performance liquid chromatographic method (HPLC) for the determination of ganoderma lucidum polysaccharide was developed, using SUGUR PARK-Ⅰ(6.5×300 mm) as the analysis column, glucan 10 000 as standard, water as mobile phase at a flow rate of 0.6 mL/min. The differential refractometer detector was used and the column temperature was 70℃. The limit of detection was 0.03 g/L. The linear regression equation was Y=109 779.6X－903.3, the correlation coefficient r=0.999 9. The method recoveries ring from 98% to 104%. Relative standard deviation was 0.12% (n=6). The method is simply, stable, suitable for the determination of ganoderma lucidum polysaccharide.

Key words: high performance liquid chromatography, differential refractometer detector, ganoderma lucidum, polysaccharide

中图分类号:

TS207

韩振泰,赵玉娟,刘惠文,李如华. 高效液相色谱法测定灵芝多糖含量[J]. , 2009, 11(S1): 65-67.

HAN Zhen-tai, ZHAO Yu-juan, LIU Hui-wen, LI Ru-hua. Determination of Ganoderma Lucidum Polysaccharide by Reversed-phase High Performance Liquid Chromatography[J]. , 2009, 11(S1): 65-67.

[1]	周明慧,伍燕湘,王松雪*. 国内外粮谷中元素含量检测标准现状与进展[J]. , 2013, 15(4): 25-30.
[2]	李志霞,聂继云*. 无损检测技术及其在果品质量安全检测中的应用[J]. , 2013, 15(4): 31-35.
[3]	李永新,黎源倩,何玲,李灿. 毛细管电泳与芯片电泳技术在转基因食品检测中的研究进展[J]. , 1, 1(1): 74-79.