ChIFN-α基因在烟草中的表达及转基因烟草对TMV的抗性

doi:10.3969/j.issn.1008-0864.2010.

›› 2010, Vol. 12 ›› Issue (1): 118-122.DOI: 10.3969/j.issn.1008-0864.2010.

ChIFN-α基因在烟草中的表达及转基因烟草对TMV的抗性

宋莉1,赵德刚1,2,田晓娥1,吴拥军2

(1.贵州省农业生物工程重点实验室, 贵阳 550025|2.贵州大学生命科学学院, 贵阳 550025)

收稿日期:2009-11-21 修回日期:2009-12-16 出版日期:2010-02-15 发布日期:2010-02-02
通讯作者: 赵德刚,教授,博士生导师,主要从事植物基因工程研究。Tel:0851-3865027; E-mail:dgzhao@gzu.edu.cn
作者简介:宋莉,讲师,博士,从事植物基因工程研究。E-mail:lpsssl@126.com
基金资助:
“十一五”国家科技支撑计划项目(2007BAD59B06);国际科技合作资助项目(2007DFA31260);国家转基因新品种培育重大专项(2008ZX08010-003)资助。

Expression of ChIFN-&alpha|in Transgenic Tobacco Causes Resistance to Tobacco Mosaic Virus

SONG Li1, ZHAO De-gang 1,2, TIAN Xiao-e1, WU Yong-jun2

(1.Guizhou Key Laboratory of Ago-bioengineering, Guiyang 550025|2.College of Life Sciences, Guizhou University, Guiyang 550025, China)

Received:2009-11-21 Revised:2009-12-16 Online:2010-02-15 Published:2010-02-02

摘要/Abstract

摘要：

动物α干扰素具有高效广谱的抗病毒作用,通过农杆菌介导法将35S驱动的干扰素基因ChIFN-α转化烟草,Southern杂交和Western杂交进行目的基因的转化、表达检测,重组蛋白的数量通过ELISA测定,对获得的转基因烟草植株进行TMV接种实验,结果表明ChIFN-α基因的转化赋予了转基因烟草对TMV的抗性,进一步的荧光定量PCR差异表达检测结果表明,该抗性可能与转基因烟草体内防卫基因PR-la、抗病N基因和信号转导MAPK基因受到上调表达有关。

关键词: 鸡α干扰素;转基因烟草;烟草花叶病毒;抗病性

Abstract:

Alpha interferon is a pleiotropic animal cytokine that possess powerful and wide-range of antiviral properties. The expression vector carrying chicken alpha interferon gene (ChIFN-α) driven by 35S promoter has been introduced into Nicotiana tabacum L. Xanthin by Agrobacterium transformation in our work. The transgenic plants were detected by Southern blot and Western blot. Recombinant ChIFN-α protein in tobacco leaf was analyzed by enzyme-linked immunosorbent assay (ELISA). The results of tobacco mosaic virus (TMV) challenge test showed that transgenic tobacco possessed certain resistance to TMV infection. And this might be related to the up-regulated expression of PR-la、N and MAPK genes in transgenic plants after TMV infection.

Key words: chicken alpha interferon, transgenic tobacco, tobacco mosaic virus, virus resistance

中图分类号:

Q812

宋莉1,赵德刚1,2,田晓娥1,吴拥军2. ChIFN-α基因在烟草中的表达及转基因烟草对TMV的抗性[J]. , 2010, 12(1): 118-122.

SONG Li1, ZHAO De-gang 1,2, TIAN Xiao-e1, WU Yong-jun2. Expression of ChIFN-&alpha|in Transgenic Tobacco Causes Resistance to Tobacco Mosaic Virus[J]. , 2010, 12(1): 118-122.

ChIFN-α基因在烟草中的表达及转基因烟草对TMV的抗性

Expression of ChIFN-&alpha|in Transgenic Tobacco Causes Resistance to Tobacco Mosaic Virus

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