鲤正、反交F2群体的AFLP遗传图谱构建及其QTL定位

›› 2010, Vol. 12 ›› Issue (1): 92-99.

鲤正、反交F2群体的AFLP遗传图谱构建及其QTL定位

王军,王成辉,刘豪,马玉清,杨新鑫

(上海海洋大学,农业部水产种质资源与利用重点开放实验室, 上海 201306)

收稿日期:2009-10-09 修回日期:2009-11-04 出版日期:2010-02-15 发布日期:2010-01-28
通讯作者: 王成辉,副教授,主要从事水产动物种质资源与遗传育种研究。Tel:021-61900439;E-mail: wangch@shou.edu.cn
作者简介:王军,硕士研究生,主要从事水产动物种质资源与遗传育种研究。
基金资助:
国家自然科学基金项目(30700622);上海市教委项目(07ZZ136);上海市重点学科建设项目(Y1101)资助。

AFLP Genetic Map Construction and QTLs Identification from F2 Reciprocal Populations in Common Carp, Cyprinus carpio L.

WANG Jun, WANG Cheng-hui, LIU Hao, MA Yu-qing, YANG Xin-xin

(Key Laboratory of Aquatic Genetic Resources and Utilization, Ministry of Agriculture, Shanghai Ocean University, Shanghai 201306, China)

Received:2009-10-09 Revised:2009-11-04 Online:2010-02-15 Published:2010-01-28

摘要/Abstract

摘要：

以高代选育的荷包红鲤和兴国红鲤的正、反交F2群体为材料,利用AFLP标记构建了鲤正、反交群体的遗传图谱,并进行了生长相关性状的QTL定位。在正交群体中,14对AFLP引物共产生542个多态性标记,其中325个标记符合3∶1的孟德尔分离比例,利用其构建的遗传图谱涵盖50个连锁群,图谱总长度3 676.1 cM,并获得了与全长、体长、体高、尾柄长和尾柄高5个生长性状相关的17个QTL,可解释表型变异的1.66%~70.49%；在反交群体中,14对AFLP引物共产生605个多态性标记,其中333个标记符合3∶1孟德尔分离比例,构建的遗传图谱也涵盖50个连锁群,图谱总长度3 943.1 cM,获得了与体重、全长、体长、体高和尾柄长5个生长性状相关的15个QTL,可解释表型变异的1.58%~63.89%。还对正、反交群体构建的遗传图谱及QTL定位差异及结果进行了初步分析和探讨。

关键词: 鲤;正反交F2群体;AFLP标记;遗传图谱;QTL

Abstract:

Taking F2 reciprocal populations derived from a diallel cross of Purse red common carp and Xingguo red common carp as material, we constructed genetic maps in the common carp (Cyprinus carpio L.) using AFLP marker and identified the growth-related quantitative trait loci (QTL). In positive mating population, 542 polymorphic markers were produced from 14 pairs of AFLP primers, in which 325 markers were accord with the ratio of 3∶1. A genetic map containing 50 linkage groups were constructed with genome length of 3 676.1 cM, and 17 QTLs related to total length, standard length, pre-dorsal height, caudal peduncle length and caudal peduncle height were achieved, which could explain 1.66% to 70.49% of the total variation. In negative mating population, 605 polymorphic markers were produced with 14 pairs of AFLP primers, in which 333 markers were accord with the ratio of 3∶1. Another genetic map containing 50 linkage groups were also constructed with genome length of 3 943.1 cM, and 15 QTLs related to body weight, total length, standard length, pre-dorsal height, caudal peduncle length were also gained, which could explain 1.58% to 63.89% of the total variation. Furthermore, this paper carried out initial analysis and discussion on genetic maps constructed from reciprocal population, the differences between QTL identification and their results.

Key words: common carp, F2 reciprocal populations, AFLP marker, genetic map, QTL

中图分类号:

S917.4

王军,王成辉,刘豪,马玉清,杨新鑫. 鲤正、反交F2群体的AFLP遗传图谱构建及其QTL定位[J]. , 2010, 12(1): 92-99.

WANG Jun, WANG Cheng-hui, LIU Hao, MA Yu-qing, YANG Xin-xin. AFLP Genetic Map Construction and QTLs Identification from F2 Reciprocal Populations in Common Carp, Cyprinus carpio L.[J]. , 2010, 12(1): 92-99.